Nature Communications (Sep 2016)

Aromatic thiol-mediated cleavage of N–O bonds enables chemical ubiquitylation of folded proteins

  • Caroline E. Weller,
  • Abhinav Dhall,
  • Feizhi Ding,
  • Edlaine Linares,
  • Samuel D. Whedon,
  • Nicholas A. Senger,
  • Elizabeth L. Tyson,
  • John D. Bagert,
  • Xiaosong Li,
  • Ohara Augusto,
  • Champak Chatterjee

DOI
https://doi.org/10.1038/ncomms12979
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 10

Abstract

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Chemical approaches to site-specifically ubiquitylate a target protein allow investigation of the biochemical effects of this modification, but they often destabilize the protein. Here, the authors report on a synthetic conjugation strategy that leads to protein ubiquitylation in non-denaturing conditions.