Optimization of phage display technology for obtaining antibodies specific to tetracycline

Abstract

Large-scale production and use of antimicrobials in human, veterinary and agricultural applications has led to antibiotic contamination of water resources. Therefore, it is relevant to develop methods for monitoring the content of antibiotics, especially in water resources. Biosensor methods are successful for the analysis of antibiotics. One of the main elements of a biosensor system is the selection of a recognition element. An alternative tool for selecting a sensitive element (recognition element) is phage display of antibodies, which makes it possible to obtain antibodies to low molecular weight antigens. The purpose of the work was to conduct research to develop and optimize the methodology for obtaining antibodies specifi c to tetracycline by using phage display technology, and to evaluate the possibility of their use for the indication of tetracycline. The work included studies of optimization of conditions for the production of phage antibodies specifi c to tetracycline, as well as the preservation of their activity during storage. The promise of using phage display technology for the production of anti-tetracycline antibodies has been shown. Using the dot immunoassay method, the possibility of using the obtained phage antibodies for tetracycline detection was shown.

Keywords

• phage display technology
• antibodies
• antibiotics