Dihydroquercetin regulates HIF-1α/AKT/NR2B signalling to improve impaired brain function in rats with metabolic syndrome
Yang Fu,
PeiPei Yuan,
Mengnan Zeng,
Qi Zhang,
Ying Hou,
Liyuan Gao,
Yaxin Wei,
Yajuan Zheng,
Weisheng Feng,
Xiaoke Zheng
Affiliations
Yang Fu
Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
PeiPei Yuan
Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
Mengnan Zeng
Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China
Qi Zhang
Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China
Ying Hou
Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China
Liyuan Gao
Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China
Yaxin Wei
Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China
Yajuan Zheng
Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China
Weisheng Feng
Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China; Corresponding author. Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Xiaoke Zheng
Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China; The Engineering and Technology Center for Chinese Medicine Development of Henan Province, Zhengzhou 450046, China; Corresponding author. Department of Pharmacy, Henan University of Chinese Medicine, Zhengzhou 450046, China.
Dihydroquercetin (DHQ) is commonly used as a dietary additive, but its activity in improving brain injury with metabolic syndrome (MS) remains known. In present study, the MS rat model was induced using 10 % fructose water. The apoptosis rate of primary brain cells was detected. The HIF-1α/AKT/NR2B signalling pathway, levels of KEAP1/NRF2, HO-1 and NQO-1 were detected. In vitro experiments were performed using H2O2-stimulated PC-12 cells. The effect of DHQ on rates of cell survival and apoptosis were detected. After silencing HIF-1α, we further elucidate the mechanism of action of DHQ. The results indicated that DHQ reduced the hyperactivity and inhibited oxidative stress via increasing the levels of HIF-1α/AKT/NR2B signalling pathway, whereas regulated KEAP1/NRF2 pathway. In vitro experiments showed that the HIF-1α plays an important role in this process. Overall, DHQ may improve impaired brain function in rats with metabolic syndrome by regulating the HIF-1α/AKT/NR2B signalling pathway.
