Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D

Warunya Chakritbudsabong; Ladawan Sariya; Sirikron Pamonsupornvichit; Rassmeepen Pronarkngver; Somjit Chaiwattanarungruengpaisan; Joao N. Ferreira; Piyathip Setthawong; Praopilas Phakdeedindan; Mongkol Techakumphu; Theerawat Tharasanit; Sasitorn Rungarunlert

doi:10.1016/j.scr.2017.08.005

Stem Cell Research (Oct 2017)

Generation of a pig induced pluripotent stem cell (piPSC) line from embryonic fibroblasts by incorporating LIN28 to the four transcriptional factor-mediated reprogramming: VSMUi001-D

Warunya Chakritbudsabong,
Ladawan Sariya,
Sirikron Pamonsupornvichit,
Rassmeepen Pronarkngver,
Somjit Chaiwattanarungruengpaisan,
Joao N. Ferreira,
Piyathip Setthawong,
Praopilas Phakdeedindan,
Mongkol Techakumphu,
Theerawat Tharasanit,
Sasitorn Rungarunlert

Affiliations

Warunya Chakritbudsabong: Department of Clinical Sciences and Public Health, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand
Ladawan Sariya: The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MOZWE), Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand
Sirikron Pamonsupornvichit: The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MOZWE), Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand
Rassmeepen Pronarkngver: The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MOZWE), Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand
Somjit Chaiwattanarungruengpaisan: The Monitoring and Surveillance Center for Zoonotic Diseases in Wildlife and Exotic Animals (MOZWE), Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand
Joao N. Ferreira: Department of Oral & Maxillofacial Surgery, Faculty of Dentistry, National University of Singapore, 11 Lower Kent Ridge Road, 119083, Singapore
Piyathip Setthawong: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
Praopilas Phakdeedindan: Biochemistry Unit, Department of Physiology, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
Mongkol Techakumphu: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
Theerawat Tharasanit: Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand
Sasitorn Rungarunlert: Department of Preclinical and Applied Animal Science, Faculty of Veterinary Science, Mahidol University, Nakhon Pathom 73170, Thailand

DOI: https://doi.org/10.1016/j.scr.2017.08.005
Journal volume & issue: Vol. 24, no. C
pp. 21 – 24

Abstract

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Pig induced pluripotent stem cell (piPSC) line was generated from embryonic fibroblast cells using retroviral transduction approaches carrying human transcriptional factors: OCT4, SOX2, KLF4, c-MYC and LIN28. The generated piPSC line, VSMUi001-D, was positive for alkaline phosphatase activity and expressed the pluripotency associated transcription factors including OCT4, SOX2, NANOG and surface markers SSEA-1, all iPSC hallmarks of authenticity. Furthermore, VSMUi001-D exhibited a normal karyotype and formed embryoid bodies in vitro and teratomas in vivo. Upon cardiac differentiation, VSMUi001-D displayed spontaneous beating and expressed cardiomyocyte markers, like cardiac Troponin T.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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