Microsphere-Based Microfluidic Device for Plasma Separation and Potential Biochemistry Analysis Applications
Hongyan Xu,
Zhangying Wu,
Jinan Deng,
Jun Qiu,
Ning Hu,
Lihong Gao,
Jun Yang
Affiliations
Hongyan Xu
Key Laboratory of Biorheological Science and Technology, Ministry of Education and Bioengineering College, Chongqing University, Chongqing 400030, China
Zhangying Wu
Key Laboratory of Biorheological Science and Technology, Ministry of Education and Bioengineering College, Chongqing University, Chongqing 400030, China
Jinan Deng
Key Laboratory of Biorheological Science and Technology, Ministry of Education and Bioengineering College, Chongqing University, Chongqing 400030, China
Jun Qiu
Department of Information, First Affiliated Hospital, Army Medical University, Chongqing 400042, China
Ning Hu
Key Laboratory of Biorheological Science and Technology, Ministry of Education and Bioengineering College, Chongqing University, Chongqing 400030, China
Lihong Gao
Chongqing Center for Drug Evaluation and Certification, Chongqing 401120, China
Jun Yang
Key Laboratory of Biorheological Science and Technology, Ministry of Education and Bioengineering College, Chongqing University, Chongqing 400030, China
The development of a simple, portable, and cost-effective plasma separation platform for blood biochemical analysis is of great interest in clinical diagnostics. We represent a plasma separation microfluidic device using microspheres with different sizes as the separation barrier. This plasma separation device, with 18 capillary microchannels, can extract about 3 μL of plasma from a 50 μL blood sample in about 55 min. The effects of evaporation and the microsphere barrier on the plasma biochemical analysis results were studied. Correction factors were applied to compensate for these two effects. The feasibility of the device in plasma biochemical analysis was validated with clinical blood samples.
