Quantification of Collagen Organization after Nerve Repair

Jacqueline S. Israel, MD; Corinne R. Esquibel, PhD; Aaron M. Dingle, PhD; Yuming Liu, PhD; Adib Keikhosravi, MS; Jane A. Pisaniello, BS; Madison A. Hesse; Sarah K. Brodnick, BS; Joseph Novello, MS; Lisa Krugner-Higby, DVM, PhD; Justin C. Williams, PhD; Kevin W. Eliceiri, PhD; Samuel O. Poore, MD, PhD

doi:10.1097/GOX.0000000000001586

Plastic and Reconstructive Surgery, Global Open (Dec 2017)

Quantification of Collagen Organization after Nerve Repair

Jacqueline S. Israel, MD,
Corinne R. Esquibel, PhD,
Aaron M. Dingle, PhD,
Yuming Liu, PhD,
Adib Keikhosravi, MS,
Jane A. Pisaniello, BS,
Madison A. Hesse,
Sarah K. Brodnick, BS,
Joseph Novello, MS,
Lisa Krugner-Higby, DVM, PhD,
Justin C. Williams, PhD,
Kevin W. Eliceiri, PhD,
Samuel O. Poore, MD, PhD

Affiliations

Jacqueline S. Israel, MD: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Corinne R. Esquibel, PhD: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Aaron M. Dingle, PhD: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Yuming Liu, PhD: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Adib Keikhosravi, MS: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Jane A. Pisaniello, BS: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Madison A. Hesse: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Sarah K. Brodnick, BS: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Joseph Novello, MS: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Lisa Krugner-Higby, DVM, PhD: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Justin C. Williams, PhD: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Kevin W. Eliceiri, PhD: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.
Samuel O. Poore, MD, PhD: From the *Division of Plastic Surgery, Department of Surgery, University of Wisconsin – Madison, Wis.; †Laboratory for Optical and Computational Instrumentation, University of Wisconsin – Madison, Wis.; ‡Department of Biomedical Engineering, College of Engineering, University of Wisconsin – Madison, Wis.; and §Department of Surgical Sciences, School of Veterinary Medicine, University of Wisconsin – Madison, Wis.

DOI: https://doi.org/10.1097/GOX.0000000000001586
Journal volume & issue: Vol. 5, no. 12
p. e1586

Abstract

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Background:. Clinical outcomes after nerve injury and repair remain suboptimal. Patients may be plagued by poor functional recovery and painful neuroma at the repair site, characterized by disorganized collagen and sprouting axons. Collagen deposition during wound healing can be intrinsically imaged using second harmonic generation (SHG) microscopy. The purpose of this study was to develop a protocol for SHG imaging of nerves and to assess whether collagen alignment can be quantified after nerve repair. Methods:. Sciatic nerve transection and epineural repair was performed in male rats. The contralateral nerves were used as intra-animal controls. Ten-millimeter nerve segments were harvested and fixed onto slides. SHG images were collected using a 20× objective on a multiphoton microscope. Collagen fiber alignment was calculated using CurveAlign software. Alignment was calculated on a scale from 0 to 1, where 1 represents perfect alignment. Statistical analysis was performed using a linear mixed-effects model. Results:. Eight male rats underwent right sciatic nerve repair using 9-0 Nylon suture. There were gross variations in collagen fiber organization in the repaired nerves compared with the controls. Quantitatively, collagen fibers were more aligned in the control nerves (mean alignment 0.754, SE 0.055) than in the repairs (mean alignment 0.413, SE 0.047; P < 0.001). Conclusions:. SHG microscopy can be used to quantitate collagen after nerve repair via fiber alignment. Given that the development of neuroma likely reflects aberrant wound healing, ex vivo and/or in vivo SHG imaging may be useful for further investigation of the variables predisposing to neuroma.

Published in Plastic and Reconstructive Surgery, Global Open

ISSN: 2169-7574 (Online)
Publisher: Wolters Kluwer
Country of publisher: United States
LCC subjects: Medicine: Surgery
Website: http://www.prsgo.com

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