Selective immunocapture and light-controlled traceless release of transiently caged proteins

Abstract

Summary: The 4,5-dimethoxy-2-nitrobenzyl (DMNB) photocaging group introduced into small biomolecules, peptides, oligonucleotides, and proteins is commonly used for spatiotemporal control of chemical and biological processes. Here, we describe the use of a DMNB-selective monoclonal antibody for non-covalent capture of chemically or biosynthetically produced proteins containing surface-exposed DMNB caging groups followed by light-controlled traceless decaging and release of the bound proteins into solution for a variety of downstream applications.For complete details on the use and execution of this protocol, please refer to Rakauskaitė et al. (2020).

Keywords

• Antibody
• Molecular/Chemical Probes
• Protein Biochemistry