Cloning, purification and characterization of human dentine matrix protein 1(DMP1)

Tarek El-Bialy; Dror Aizenbud; Hamid Sadeghi; Paul Scott; Itay Aizenbud

Saudi Dental Journal (Jul 2023)

Cloning, purification and characterization of human dentine matrix protein 1(DMP1)

Tarek El-Bialy,
Dror Aizenbud,
Hamid Sadeghi,
Paul Scott,
Itay Aizenbud

Affiliations

Tarek El-Bialy: Division Orthodontics, School of Dentistry, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, AB, Canada; Corresponding author.
Dror Aizenbud: Department of Orthodontics and Craniofacial Anomalies, School of Graduate Dentistry, Rambam Health Care Center and Technion Haifa, Israel
Hamid Sadeghi: Department of Dentistry, University of Alberta, Edmonton, Canada
Paul Scott: Department of Biochemistry, University of Alberta, Edmonton Canada
Itay Aizenbud: Institute of Dental Sciences, Faculty of Dental Medicine, Hebrew University, Jerusalem; Medical Corps, Israel Defense Forces, Tel-Hashomer, Israel

Journal volume & issue: Vol. 35, no. 5
pp. 553 – 558

Abstract

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Introduction: Human teeth are composed mainly of dentin, formed by the odontoblasts. Dentin matrix protein 1 (DMP1) is one of odontoblast differentiation's most important growth factors. Human DMP1 has yet to be completely identified or studied. This study aimed to clone and characterize human DMP1. Materials and methods: The DMP1 gene sequence was prepared and cloned by transfection of human 293 cells. Results: The recombinant DMP1 was purified and characterized. Conclusion: The results suggested its future use in dental tissue regeneration and tissue engineering.

Published in Saudi Dental Journal

ISSN: 1013-9052 (Print); 1658-3558 (Online)
Publisher: Elsevier
Country of publisher: Saudi Arabia
LCC subjects: Medicine: Dentistry
Website: https://www.journals.elsevier.com/saudi-dental-journal/

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Abstract

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