Natterin-like depletion by CRISPR/Cas9 impairs zebrafish (Danio rerio) embryonic development

Abstract

Highlights The Natterin family was discovered in the venom of the fish Thalassophryne nattereri. The zebrafish genome encodes eleven natterin-like genes. Natterin-like might be a novel fish-specific defense molecule. Natterin-like proteins are thought to be pore-forming molecules. Reverse genetic study and phenotypic characterization suggests natterin-like genes may have roles in zebrafish development.

Keywords

• Natterin proteins family
• Danio rerio
• Embryogenesis
• CRISPR/Cas9
• Phenotype-based screening
• Physiological functions