Composite brushite-monetite-newberyite cements loaded with vancomycin and their efficiency against infections of: In vitro and in vivo research
Yulia Lukina,
Sergey Kotov,
Aleksandr Senyagin,
Leonid Bionyshev-Abramov,
Natalya Serejnikova,
Rostislav Chelmodeev,
Alexander Tavtorkin,
Maria Ryndyk,
Dmitriiy Smolentsev,
Sergey Sivkov,
Tatiana Safronova
Affiliations
Yulia Lukina
Priorov Central Institute for Trauma and Orthopedics Ministry of Health of the Russian Federation, ul. Priorova 10, Moscow, 127299, Russia; Faculty of Digital Technologies and Chemical Engineering, Mendeleev University of Chemical Technology of Russia, Miusskaya pl. 9, Moscow, 125047, Russia; Corresponding author. Priorov Central Institute for Trauma and Orthopedics Ministry of Health of the Russian Federation, ul. Priorova 10, Moscow, 127299, Russia.
Sergey Kotov
Building materials physico-chemical Testing Department of the Experimental Tests preparation and Carry Out Department № 29 Research Institute of Concrete and Reinforced Concrete (NIIZNB) Named after A.A. Gvozdev, JSC Research Center of Construction, 2nd Institutskaya ul., 6, Moscow, 109428, Russia
Aleksandr Senyagin
Department of Medicine, Peoples Friendship University of Russia Name after Patrice Lumumba (RUDN University), Miklukho-Maklaya 6, 117198, Moscow, Russia
Leonid Bionyshev-Abramov
Priorov Central Institute for Trauma and Orthopedics Ministry of Health of the Russian Federation, ul. Priorova 10, Moscow, 127299, Russia
Natalya Serejnikova
Priorov Central Institute for Trauma and Orthopedics Ministry of Health of the Russian Federation, ul. Priorova 10, Moscow, 127299, Russia; Institute for Regenerative Medicine, Sechenov First Moscow State Medical University, Trubetskaya, 8, 119991, Moscow, Russia
Rostislav Chelmodeev
Priorov Central Institute for Trauma and Orthopedics Ministry of Health of the Russian Federation, ul. Priorova 10, Moscow, 127299, Russia; Power Engineering Department, Bauman Moscow State Technical University, ul. 2 Baumanskaya, 5, b.1, Moscow, 105005, Russia
Alexander Tavtorkin
A. V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninskii pr., 29, Moscow, 119991, Russia
Maria Ryndyk
A. V. Topchiev Institute of Petrochemical Synthesis, Russian Academy of Sciences, Leninskii pr., 29, Moscow, 119991, Russia; Faculty of Chemistry, National Research University Higher School of Economics, 101100 Moscow, Russian Federation
Dmitriiy Smolentsev
Priorov Central Institute for Trauma and Orthopedics Ministry of Health of the Russian Federation, ul. Priorova 10, Moscow, 127299, Russia
Sergey Sivkov
Faculty of Technology of Inorganic Substances and High-Temperature Materials, Mendeleev University of Chemical Technology of Russia, Miusskaya pl. 9, Moscow, 125047, Russia
Tatiana Safronova
Department of Materials Science, Lomonosov Moscow State University, Building, 73, Leninskie Gory, 1, 119991, Moscow, Russia; Department of Chemistry, Lomonosov Moscow State University, Building, 3, Leninskie Gory, 1, 119991 Moscow, Russia
Composite cements containing brushite, monetite and newberyite as dominating phases were created and used as drug (vancomycin) carriers. Brushite-monetite-newberyite cement with variable composition was obtained by replacing Ca2+ ions with Mg2+ in the initial cement component. Starting powder mixtures for cement preparation synthesized via solid state reaction consisted of MgxCa(3-х)(PO4)2 (x = 0, 0.75, 1.5, 2.25, 3), monocalcium phosphate monohydrate Ca(H2PO4)2·H2O with and without vancomycin as antibacterial agent. 100 mg of powder of a pharmaceutical substance per 1 g of the dry cement powder was added before cement sample preparation. Mixing liquids used were water solutions containing sodium pyrophosphate hexahydrate Na4P2O7·10H2O and citric acid monohydrate C6H8O7·H2O as set-retarding additives. An increase in the concentration of Mg2+ ions entail an increase in pH during preparation and a decrease in porosity of cement sample. Incorporation of vancomycin at the stage of preparation of the cement mixture does not affect the strength characteristics, porosity and pH of the cement stone. The prolongation of the release of vancomycin depends on the composition and characteristics of the cement stone. The activity of vancomycin against Staphylloccoscus aureus and Escherichia coli the process of hydration of cement and its dissolution was confirmed by appropriate tests in vitro. In vivo studies confirm the biocompatibility, resorbability of cements, and the effectiveness of their use as carriers of vancomycin in the treatment of purulent-septic inflammation.
