Frontiers in Cell and Developmental Biology (Jul 2021)

Cell Division Cycle 5-Like Regulates Metaphase-to-Anaphase Transition in Meiotic Oocyte

  • Hong-Yong Zhang,
  • Hong-Yong Zhang,
  • Hong-Yong Zhang,
  • Jian Li,
  • Ying-Chun Ouyang,
  • Tie-Gang Meng,
  • Tie-Gang Meng,
  • Chun-Hui Zhang,
  • Wei Yue,
  • Qing-Yuan Sun,
  • Wei-Ping Qian,
  • Wei-Ping Qian

DOI
https://doi.org/10.3389/fcell.2021.671685
Journal volume & issue
Vol. 9

Abstract

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The quality of oocytes is a vital factor for embryo development. Meiotic progression through metaphase I usually takes a relatively long time to ensure correct chromosome separation, a process that is critical for determining oocyte quality. Here, we report that cell division cycle 5-like (Cdc5L) plays a critical role in regulating metaphase-to-anaphase I transition during mouse oocyte meiotic maturation. Knockdown of Cdc5L by small interfering RNA injection did not affect spindle assembly but caused metaphase I arrest and subsequent reduced first polar body extrusion due to insufficient anaphase-promoting complex/cyclosome activity. We further showed that Cdc5L could also directly interact with securin, and Cdc5L knockdown led to a continuous high expression level of securin, causing severely compromised meiotic progression. The metaphase-to-anaphase I arrest caused by Cdc5L knockdown could be rescued by knockdown of endogenous securin. In summary, we reveal a novel role for Cdc5L in regulating mouse oocyte meiotic progression by interacting with securin.

Keywords