Evaluation of toxicological and antioxidant potential of Nardostachys jatamansi in reversing haloperidol-induced catalepsy in rats

Abstract

A S Rasheed1, S Venkataraman1, K N Jayaveera2, A Mohammed Fazil3, K J Yasodha3, M A Aleem3, M Mohammed3, Z Khaja3, B Ushasri3, H A Pradeep3, M Ibrahim31Department of Pharmacology and Toxicology, C L Baid Metha College of Pharmacy, Jyothi Nagar, Thoraipakkam, Chennai, Tamil Nadu, India; 2Department of Chemistry, Jawaharlal Nehru Technology University, Anantapur, Andhra Pradesh, India; 3Nizam Institute of Pharmacy and Research Center, Deshmukhi, Pochampally (M), Near Ramoji Film City, Nalgonda, (AP), IndiaAbstract: An aqueous root extract from Nardostachys jatamansi was investigated for its antioxidant and anticataleptic effects in the haloperidol-induced catalepsy rat model of the disease by measuring various behavioral and biochemical parameters. Catalepsy was induced by administration of haloperidol (1 mg/kg, ip) in male albino rats. A significant (P < 0.01) reduction in the cataleptic scores were observed in all the drug-treated groups as compared to the haloperidol-treated group; with maximum reduction observed in the Nardostachys jatamansi (250 and 500 mg/kg body weight) administered group. To estimate biochemical parameters: the generation of thiobarbituric acid reactive substances (TBARS); reduced glutathione (GSH) content and glutathione-dependent enzymes; catalase; and superoxide dismutase (SOD), in the brain were assessed. Haloperidol administration increased generation of TBARS and significantly reduced GSH, which were restored to near normal level with the Nardostachys jatamansi treatment. Catalase and SOD levels were also increased to normal levels, having been reduced significantly by haloperidol administration. Our findings of behavioral studies and biochemical estimations show that Nardostachys jatamansi reversed the haloperidol-induced catalepsy in rats. We conclude that the antioxidant potential has contributed to the reduction in the oxidative stress and catalepsy induced by haloperidol administration.Keywords: Nardostachys jatamansi, antioxidant, toxicological, haloperidol and catalepsy