Phenolic profiling and bioactivity assessment of in vitro propagated Psidium cattleianum Sabine: A promising study
Eman M. El-Deeb,
Heba E. Elsayed,
Hanaa B. Ateya,
Hussein S. Taha,
Mohamed R. Elgindi,
Doaa Abouelenein,
Giovanni Caprioli,
Kuei-Hung Lai,
Ahmed M. Mustafa,
Fatma A. Moharram
Affiliations
Eman M. El-Deeb
Department of Pharmacognosy, Faculty of Pharmacy, October 6 University, Giza, Egypt
Heba E. Elsayed
Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, Egypt
Hanaa B. Ateya
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, Helwan University, Cairo, Egypt
Hussein S. Taha
Department of Plant Biotechnology, Biotechnology Research Institute, National Research Centre, Cairo, Egypt
Mohamed R. Elgindi
Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, Egypt
Doaa Abouelenein
School of Pharmacy, University of Camerino, via Sant’ Agostino 1, Camerino, Italy
Giovanni Caprioli
School of Pharmacy, University of Camerino, via Sant’ Agostino 1, Camerino, Italy
Kuei-Hung Lai
Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei, Taiwan; PhD Program in Clinical Drug Development of Herbal Medicine, College of Pharmacy, Taipei Medical University, Taipei, Taiwan; Traditional Herbal Medicine Research Center, Taipei Medical University Hospital, Taipei, Taiwan; Corresponding author. Graduate Institute of Pharmacognosy, College of Pharmacy, Taipei Medical University, Taipei, 11031, Taiwan.
Ahmed M. Mustafa
School of Pharmacy, University of Camerino, via Sant’ Agostino 1, Camerino, Italy; Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt
Fatma A. Moharram
Department of Pharmacognosy, Faculty of Pharmacy, Helwan University, Cairo, Egypt
Psidium cattleianum Sabine (strawberry guava) is an evergreen shrub that is grown as a fruiting hedge and has received significant consideration in the food and pharmaceutical disciplines. This study aims to set a promising protocol for in vitro propagation of P. cattleianum, along with profiling the phenolic content of the original plant (OP), induced callus (IC), and regenerated plantlets (RP) extracts, ultimately, evaluating their anti-inflammatory, antioxidant, and anticancer potential. Seeds were treated with commercial bleaching, HCl, and H2O2 to enhance the germination percentage and minimize the contamination percentage. Culturing sterilized leaf explants onto Murashige and Skoog (MS) medium supplemented with benzyl adenine (BA), 2,4-dichloro phenoxy acetic acid, and kinetin showed the best callus induction, while supplementation of MS media with BA, adenine sulfate, naphthalene acetic acid, and gibberellic acid activated regeneration. Augmentation of MS media with indol-3-butyric acid recorded the maximum rooting percentage. Finally, the obtained rooted shoots were successfully acclimatized in sand and peat moss soil. HPLC-MS/MS profiles of OP, RP, and IC showed a variety of phenolic metabolites. IC extract decreased the viability of MCF-7, HepG2, and K-562 cancer cell lines. Also, OP exhibits strong antioxidant activity. P. cattleianum and its RP are profound sources of phenolic compounds promoted for promising applications in the food and pharmaceutical industries.
