Design and Production of A Wound Cover Containing Essence of Ajwain (Trachyspermum) by Nanoliposome Technique, and Assessment of Its Physical, Chemical, Antibacterial and Cytotoxicity Properties

Abstract

Background and Aim: The aim of this study was to assess the applied characteristics of wound covers containing nanoliposomic essential oil of ajwain, with suitable antimicrobial properties and lack of cytotoxicity. Materials and Methods: Liposomal formulations of the ajwain essential oil containing DSPE-PEG, cholesterol, span60 and SPC80 were prepared using a thin layer method. The rooting and spray methods on a cellulose fabric were used to produce skin wound cover. In addition to in vitro intracellular penetration and measurement of minimum inhibitory concentration of the product, textile characteristics, antimicrobial activity and 96 hours release of the essence in the wound cover were studied. Ethical Considerations: In this study, all principles of research ethics were considered. Findings: The loading efficiency of the liposomal formulation was more than 85%. The small particle dispersion index (PDI = 0.02) in the form of the PEGylated formulation indicates optimal dispersion of the particles which reduces the buildup of the drug in the cutaneous application. The standard AATCC microbial test showed inhibitory effect of the wound cover on bacteria, especially E. coli. Textile tests indicated acceptable properties of the produced wound cover, too. Conclusion: Altogether, this wound cover showed acceptable features in combating the two selected bacteria responsible for infectious skin ulcers.

Keywords

• Ajwain
• Cytotoxicity
• Essence
• Liposome
• Textile
• Wound cover