Combination of Two Kinds of Medicated Microparticles Based on Hyaluronic Acid or Chitosan for a Wound Healing Spray Patch
Angela Fabiano,
Chiara Migone,
Luca Cerri,
Anna Maria Piras,
Andrea Mezzetta,
Giuseppantonio Maisetta,
Semih Esin,
Giovanna Batoni,
Rossella Di Stefano,
Ylenia Zambito
Affiliations
Angela Fabiano
Department of Pharmacy, University of Pisa, Via Bonanno 33, 56126 Pisa, Italy
Chiara Migone
Department of Pharmacy, University of Pisa, Via Bonanno 33, 56126 Pisa, Italy
Luca Cerri
Department of Pharmacy, University of Pisa, Via Bonanno 33, 56126 Pisa, Italy
Anna Maria Piras
Department of Pharmacy, University of Pisa, Via Bonanno 33, 56126 Pisa, Italy
Andrea Mezzetta
Department of Pharmacy, University of Pisa, Via Bonanno 33, 56126 Pisa, Italy
Giuseppantonio Maisetta
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy
Semih Esin
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy
Giovanna Batoni
Department of Translational Research and New Technologies in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy
Rossella Di Stefano
Cardiovascular Research Laboratory, Department of Surgery, Medical, Molecular, and Critical Area Pathology, University of Pisa, Via Paradisa 2, 56100 Pisa, Italy
Ylenia Zambito
Department of Pharmacy, University of Pisa, Via Bonanno 33, 56126 Pisa, Italy
Olive leaves extract (OLE) has been extensively studied as antioxidant and antibiotic and these characteristics make it particularly interesting for use on wounds. For this reason, the aim of this study was to introduce OLE in microparticles (MP) of hyaluronic acid (MPHA-OLE) or chitosan (MPCs-OLE) to obtain a spray patch for the treatment of wounds in anatomical areas that are difficult to protect with traditional patches. The MP were characterized for particle size and ability to protect OLE from degradation, to absorb water from wound exudate, to control OLE release from MP. The MPHA and MPCs medicated or not and mixtures of the two types in different proportions were studied in vitro on fibroblasts by the scratch wound healing assay. The MP size was always less than 5 µm, and therefore, suitable for a spray patch. The MPCs-OLE could slow down the release of OLE therefore only about 60% of the polyphenols contained in it were released after 4 h. Both MPHA and MPCs could accelerate wound healing. A 50% MPHA-OLE-50% MPCs-OLE blend was the most suitable for accelerating wound healing. The MPHA-OLE-MPCs-OLE blends studied in this work were shown to have the characteristics suitable for a spray patch, thus giving a second life to the waste products of olive growers.
