Frontiers in Immunology (Oct 2021)
Mannan Oligosaccharides Application: Multipath Restriction From Aeromonas hydrophila Infection in the Skin Barrier of Grass Carp (Ctenopharyngodon idella)
- Zhiyuan Lu,
- Lin Feng,
- Lin Feng,
- Lin Feng,
- Wei-Dan Jiang,
- Wei-Dan Jiang,
- Wei-Dan Jiang,
- Pei Wu,
- Pei Wu,
- Pei Wu,
- Yang Liu,
- Yang Liu,
- Yang Liu,
- Jun Jiang,
- Jun Jiang,
- Jun Jiang,
- Sheng-Yao Kuang,
- Sheng-Yao Kuang,
- Ling Tang,
- Ling Tang,
- Shu-Wei Li,
- Shu-Wei Li,
- Xiang-An Liu,
- Cheng-Bo Zhong,
- Xiao-Qiu Zhou,
- Xiao-Qiu Zhou,
- Xiao-Qiu Zhou
Affiliations
- Zhiyuan Lu
- Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China
- Lin Feng
- Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China
- Lin Feng
- Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Lin Feng
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, China
- Wei-Dan Jiang
- Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China
- Wei-Dan Jiang
- Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Wei-Dan Jiang
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, China
- Pei Wu
- Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China
- Pei Wu
- Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Pei Wu
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, China
- Yang Liu
- Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China
- Yang Liu
- Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Yang Liu
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, China
- Jun Jiang
- Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China
- Jun Jiang
- Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Jun Jiang
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, China
- Sheng-Yao Kuang
- Sichuan Animal Science Academy, Sichuan Animtech Feed Co. Ltd, Chengdu, China
- Sheng-Yao Kuang
- Animal Breeding and Genetics Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Academy of Animal Science, Chengdu, China
- Ling Tang
- Sichuan Animal Science Academy, Sichuan Animtech Feed Co. Ltd, Chengdu, China
- Ling Tang
- Animal Breeding and Genetics Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Academy of Animal Science, Chengdu, China
- Shu-Wei Li
- Sichuan Animal Science Academy, Sichuan Animtech Feed Co. Ltd, Chengdu, China
- Shu-Wei Li
- Animal Breeding and Genetics Key Laboratory of Sichuan Province, Animal Nutrition Institute, Sichuan Academy of Animal Science, Chengdu, China
- Xiang-An Liu
- Sichuan Animal Science Academy, Sichuan Animtech Feed Co. Ltd, Chengdu, China
- Cheng-Bo Zhong
- Sichuan Animal Science Academy, Sichuan Animtech Feed Co. Ltd, Chengdu, China
- Xiao-Qiu Zhou
- Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China
- Xiao-Qiu Zhou
- Fish Nutrition and Safety Production University Key Laboratory of Sichuan Province, Sichuan Agricultural University, Chengdu, China
- Xiao-Qiu Zhou
- Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, China
- DOI
- https://doi.org/10.3389/fimmu.2021.742107
- Journal volume & issue
-
Vol. 12
Abstract
The objective of this study was to evaluate the efficacy of dietary Mannan oligosaccharides (MOS) supplementation on skin barrier function and the mechanism of on-growing grass carp (Ctenopharyngodon idella). Five hundred forty grass carp were fed for 60 days from the growing stage with six different levels of MOS diets (0, 200, 400, 600, 800, and 1,000 mg kg-1). At the end of the growth trial, the 14-day Aeromonas hydrophila challenge experiment has proceeded. The obtained data indicate that MOS could (1) decline skin lesion morbidity after being challenged by the pathogenic bacteria; (2) maintain physical barrier function via improving antioxidant ability, inhibiting excessive apoptosis, and strengthening the tight junction between the epithelial cell and the related signaling pathway (Nrf2/Keap1, p38MAPK, and MLCK); and (3) regulate immune barrier function by modulating the production of antimicrobial compound and expression of involved cytokines and the related signaling pathway (TOR and NFκB). Finally, we concluded that MOS supplementation reinforced the disease resistance and protected the fish skin barrier function from Aeromonas hydrophila infection.
Keywords
- mannan oligosaccharides
- antioxidant
- apoptosis
- tight junction
- skin immune
- grass carp (Ctenopharyngodon idella)
