Targeted Isolation of Xenicane Diterpenoids From Taiwanese Soft Coral <i>Asterospicularia laurae</i>
Yu-Chi Lin,
Yi-Jen Chen,
Shu-Rong Chen,
Wan-Ju Lien,
Hsueh-Wei Chang,
Yu-Liang Yang,
Chia-Ching Liaw,
Jui-Hsin Su,
Ching-Yeu Chen,
Yuan-Bin Cheng
Affiliations
Yu-Chi Lin
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804351, Taiwan
Yi-Jen Chen
Department of Fragrance and Cosmetic Science, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807378, Taiwan
Shu-Rong Chen
Graduate Institute of Natural Products, Center for Natural Product Research and Development, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807378, Taiwan
Wan-Ju Lien
Department of Biomedical Science and Environmental Biology, PhD Program in Life Science, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Hsueh-Wei Chang
Department of Biomedical Science and Environmental Biology, PhD Program in Life Science, College of Life Science, Kaohsiung Medical University, Kaohsiung 80708, Taiwan
Yu-Liang Yang
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804351, Taiwan
Chia-Ching Liaw
Division of Chinese Materia Medica Development, National Research Institute of Chinese Medicine, Taipei 11221, Taiwan
Jui-Hsin Su
Graduate Institute of Marine Biology, National Dong Hwa University, Pingtung 944401, Taiwan
Ching-Yeu Chen
Department of Physical Therapy, Tzu-Hui Institute of Technology, Pingtung 92641, Taiwan
Yuan-Bin Cheng
Department of Marine Biotechnology and Resources, National Sun Yat-sen University, Kaohsiung 804351, Taiwan
Application of LC-MS/MS-based molecular networking indicated the ethanol extract of octocoral Asterospicularia laurae is a potential source for the discovery of new xenicane derivatives. A natural product investigation of this soft coral resulted in the isolation of four new xenicane diterpenoids, asterolaurins O–R (1–4), together with six known compounds, xeniolide-A (5), isoxeniolide-A (6), xeniolide-B (7), 7,8-epoxyxeniolide-B (8), 7,8-oxido-isoxeniolide-A (9), and 9-hydroxyxeniolide-F (10). The structures of isolated compounds were characterized by employing spectroscopic analyses, including 2D-NMR (COSY, HMQC, HMBC, and NOESY) and high-resolution electrospray ionization mass spectrometry (HRESIMS). Asterolaurin O is the first case of brominated tricarbocyclic type floridicin in the family Xeniidae. Concerning bioactivity, the cytotoxic activity of those isolates was evaluated. As a result, compounds 1 and 2 demonstrated a selective cytotoxic effect against the MCF-7 cell line at IC50 of 14.7 and 25.1 μM, respectively.
