Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels

Bernhard Illes; Hanna Engelke

STAR Protocols (Sep 2021)

Protocol for photoactivation of YAP in cancer cell spheroids embedded in collagen gels

Bernhard Illes,
Hanna Engelke

Affiliations

Bernhard Illes: Department of Chemistry and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität München, Butenandtstr. 11, 81377 Munich, Germany; Corresponding author
Hanna Engelke: Department of Chemistry and Center for NanoScience (CeNS), Ludwig-Maximilians-Universität München, Butenandtstr. 11, 81377 Munich, Germany; Institute of Pharmaceutical Sciences, Department of Pharmaceutical Chemistry, University of Graz, Humboldtstr. 46, 8010 Graz, Austria; Corresponding author

Journal volume & issue: Vol. 2, no. 3
p. 100657

Abstract

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Summary: This protocol describes the necessary preparations and procedures to photo-activate Yes-associated protein (YAP) with optoYAP in cancer cell spheroids in 3D collagen matrices. We detail steps for immunofluorescent staining of the resulting YAP-activated HeLa spheroids. In addition, we describe handling of optoYAP on 2D substrates. While this protocol focuses on the use of optoYAP in 3D HeLa cell culture, it can be modified for other cell types.For complete details on the use and execution of this protocol, please refer to Illes et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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