In Vitro Destruction of Pathogenic Bacterial Biofilms by Bactericidal Metallic Nanoparticles via Laser-Induced Forward Transfer
Alena Nastulyavichus,
Eteri Tolordava,
Andrey Rudenko,
Darya Zazymkina,
Pavel Shakhov,
Nikolay Busleev,
Yulia Romanova,
Andrey Ionin,
Sergey Kudryashov
Affiliations
Alena Nastulyavichus
Laboratory of Laser Nanophysics and Biomedicine, P.N. Lebedev Physical Institute of the Russian Academy of Sciences, 119991 Moscow, Russia
Eteri Tolordava
Laboratory of Laser Nanophysics and Biomedicine, P.N. Lebedev Physical Institute of the Russian Academy of Sciences, 119991 Moscow, Russia
Andrey Rudenko
Laboratory of Laser Nanophysics and Biomedicine, P.N. Lebedev Physical Institute of the Russian Academy of Sciences, 119991 Moscow, Russia
Darya Zazymkina
Laboratory of Laser Nanophysics and Biomedicine, P.N. Lebedev Physical Institute of the Russian Academy of Sciences, 119991 Moscow, Russia
Pavel Shakhov
Laboratory of Laser Nanophysics and Biomedicine, P.N. Lebedev Physical Institute of the Russian Academy of Sciences, 119991 Moscow, Russia
Nikolay Busleev
Laboratory of Laser Nanophysics and Biomedicine, P.N. Lebedev Physical Institute of the Russian Academy of Sciences, 119991 Moscow, Russia
Yulia Romanova
Laboratory of Genetic Engineering of Pathogenic Microorganisms, N.F. Gamaleya Federal Research Centre of Epidemiology and Microbiology, 123098 Moscow, Russia
Andrey Ionin
Laboratory of Laser Nanophysics and Biomedicine, P.N. Lebedev Physical Institute of the Russian Academy of Sciences, 119991 Moscow, Russia
Sergey Kudryashov
Laboratory of Laser Nanophysics and Biomedicine, P.N. Lebedev Physical Institute of the Russian Academy of Sciences, 119991 Moscow, Russia
A novel, successful method of bactericidal treatment of pathogenic bacterial biofilms in vitro by laser-induced forward transfer of metallic nanoparticles from a polyethylene terephthalate polymeric substrate was suggested. Transferred nanoparticles were characterized by scanning and transmission electron microscopy, energy-dispersive X-ray and Raman spectroscopy. The antibacterial modality of the method was tested on Gram-positive (Staphylococcus aureus) and Gram-negative (Pseudomonas Aeruginosa) bacterial biofilms in vitro, revealing their complete destruction. The proposed simple, cost-effective and potentially mobile biofilm treatment method demonstrated its high and broad bactericidal efficiency.
