Development of caprine chimeric embryos reconstructed through ES-tetraploid complementation assay

Abstract

The aim of the present study was to evaluate the development of caprine chimeric embryos in different culture media, viz. RVCL and mCR2aa + 10% FBS. Inner cell mass (ICM) from hatched blastocysts of parthenogenetic activated embryos were used to produce ES cell-like cells (103) while 4-cell embryos obtained from IVF were utilized to produce tetraploid embryos (231). The aggregates prepared were randomly divided into 2 groups, viz. Gr 1 (RVCL) (53) and Gr 2 (mCR2aa +10% FBS) (50) followed by culture in humidified atmosphere of 5% CO2 at 38.5°C in a CO2 incubator. Outcome measures were aggregation, 8–16 cell, morula and blastocyst formation. The percentage of aggregation, 8–16 cell, morula and blastocyst in Gr 1 (RVCL) was 83.33±6.00%, 42.24±7.71%,29.59±7.64% and 11.49±5.36%, respectively while the percentage of aggregation, 8–16 cell, morula and blastocyst in Gr 2 (mCR2aa +10% FBS) was 91.66±4.32%, 54.76±7.75%, 22.32±5.75% and 14.58±5.18%, respectively. In conclusion, both the media supported the development of chimeric embryos up to blastocyst.

Keywords

• Caprine
• Chimeric embryos
• Culture media
• ES-tetraploid complementation assay