Frontiers in Immunology (May 2017)

Assessment of Spanish Panel Reactive Antibody Calculator and Potential Usefulness

  • Esther Asensio,
  • Esther Asensio,
  • Marcos López-Hoyos,
  • Marcos López-Hoyos,
  • Íñigo Romón,
  • Jesús Ontañón,
  • David San Segundo,
  • David San Segundo

DOI
https://doi.org/10.3389/fimmu.2017.00540
Journal volume & issue
Vol. 8

Abstract

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Background and objectivesThe calculated panel reactive of antibodies (cPRAs) necessary for kidney donor-pair exchange and highly sensitized programs are estimated using different panel reactive antibody (PRA) calculators based on big enough samples in Eurotransplant (EUTR), United Network for Organ Sharing (UNOS), and Canadian Transplant Registry (CTR) websites. However, those calculators can vary depending on the ethnic they are applied. Here, we develop a PRA calculator used in the Spanish Program of Transplant Access for Highly Sensitized patients (PATHI) and validate it with EUTR, UNOS, and CTR calculators.MethodsThe anti-human leukocyte antigen (HLA) antibody profile of 42 sensitized patients on waiting list was defined, and cPRA was calculated with different PRA calculators.ResultsDespite different allelic frequencies derived from population differences in donor panel from each calculator, no differences in cPRA between the four calculators were observed. The PATHI calculator includes anti-DQA1 antibody profiles in cPRA calculation; however, no improvement in total cPRA calculation of highly sensitized patients was demonstrated.Interpretation and conclusionThe PATHI calculator provides cPRA results comparable with those from EUTR, UNOS, and CTR calculators and serves as a tool to develop valid calculators in geographical and ethnic areas different from Europe, USA, and Canada.

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