Nature Communications (Aug 2016)

Non-homologous DNA increases gene disruption efficiency by altering DNA repair outcomes

  • C. D. Richardson,
  • G. J. Ray,
  • N. L. Bray,
  • J. E. Corn

DOI
https://doi.org/10.1038/ncomms12463
Journal volume & issue
Vol. 7, no. 1
pp. 1 – 7

Abstract

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CRISPR-Cas9 mediated gene editing has begun to revolutionize molecular biology, but editing efficiencies can vary greatly between reagents. The authors show that the addition of single-stranded non-homologous DNA stimulates gene disruption by favouring error-prone DNA repair.