Hyaluronan-modified transfersomes based hydrogel for enhanced transdermal delivery of indomethacin
Ming Yuan,
Jiangxiu Niu,
Qinghan Xiao,
Huiyuan Ya,
Yansong Zhang,
Yanli Fan,
Lingmei Li,
Xueke Li
Affiliations
Ming Yuan
College of Food and Drug, Henan Functional Cosmetics Engineering & Technology Research Center, Luoyang Normal University, Luoyang, People’s Republic of China
Jiangxiu Niu
College of Food and Drug, Henan Functional Cosmetics Engineering & Technology Research Center, Luoyang Normal University, Luoyang, People’s Republic of China
Qinghan Xiao
College of Food and Drug, Henan Functional Cosmetics Engineering & Technology Research Center, Luoyang Normal University, Luoyang, People’s Republic of China
Huiyuan Ya
College of Food and Drug, Henan Functional Cosmetics Engineering & Technology Research Center, Luoyang Normal University, Luoyang, People’s Republic of China
Yansong Zhang
College of Food and Drug, Henan Functional Cosmetics Engineering & Technology Research Center, Luoyang Normal University, Luoyang, People’s Republic of China
Yanli Fan
College of Food and Drug, Henan Functional Cosmetics Engineering & Technology Research Center, Luoyang Normal University, Luoyang, People’s Republic of China
Lingmei Li
College of Food and Drug, Henan Functional Cosmetics Engineering & Technology Research Center, Luoyang Normal University, Luoyang, People’s Republic of China
Xueke Li
College of Food and Drug, Henan Functional Cosmetics Engineering & Technology Research Center, Luoyang Normal University, Luoyang, People’s Republic of China
Hyaluronic acid (HA), as a hygroscopic and biocompatible molecule, has displayed unique permeation enhancement in transdermal delivery systems. Hence, indomethacin (IND) was encapsulated in HA-modified transfersomes (IND-HTs) to enhance transdermal IND delivery to reduce adverse effects in this study. The physiochemical properties of IND-HTs were characterized. Results showed that the prepared IND-HTs were spherical and revealed good entrapment efficiency (87.88 ± 2.03%), with a nanometric particle size (221.8 ± 93.34 nm). Then, IND-HTs were further incorporated into a carbopol 940 hydrogel (IND-HTs/Gel) to prolong retention capacity on the skin. The in vitro release and skin permeation experiments of IND-HTs/Gel were carried out with the Franz diffusion cells. It was found that IND-HTs/Gel exhibited sustained drug release, as well as superior drug permeation and flux across the skin. Confocal laser scanning microscopy showed improved penetration of HTs/Gel with a wider distribution and higher fluorescence intensity. The hematoxylin–eosin stained showed that HA improved the transdermal effect by changing the microstructure of skin layers and decreasing skin barrier function. In addition, IND-HTs/Gel showed significant analgesic activity in hot plate test and no potentially hazardous skin irritation. This study indicated that the developed IND-HTs/Gel could be a promising alternative to conventional oral delivery of IND by topical administration.
