Changing oxidoreduction potential to improve water-soluble yellow pigment production with Monascus ruber CGMCC 10910

Tao Huang; Hailing Tan; Fangju Lu; Gong Chen; Zhenqiang Wu

doi:10.1186/s12934-017-0828-0

Microbial Cell Factories (Nov 2017)

Changing oxidoreduction potential to improve water-soluble yellow pigment production with Monascus ruber CGMCC 10910

Tao Huang,
Hailing Tan,
Fangju Lu,
Gong Chen,
Zhenqiang Wu

Affiliations

Tao Huang: School of Biology and Biological Engineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology
Hailing Tan: School of Biology and Biological Engineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology
Fangju Lu: School of Biology and Biological Engineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology
Gong Chen: School of Biology and Biological Engineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology
Zhenqiang Wu: School of Biology and Biological Engineering, Guangdong Provincial Key Laboratory of Fermentation and Enzyme Engineering, South China University of Technology

DOI: https://doi.org/10.1186/s12934-017-0828-0
Journal volume & issue: Vol. 16, no. 1
pp. 1 – 12

Abstract

Read online

Abstract Background Monascus pigments are widely used in the food and pharmaceutical industries due to their safety to human health. Our previous study found that glucose concentration induced extracellular oxidoreduction potential (ORP) changes could influence extracellular water-soluble yellow pigment production by Monascus ruber CGMCC 10910 in submerged fermentation. In this study, H2O2 and dithiothreitol (DTT) were used to change the oxidoreduction potential for investigating the effects of oxidative or reductive substances on Monascus yellow pigment production by Monascus ruber CGMCC 10910. Results The extracellular ORP could be controlled by H2O2 and DTT. Both cell growth and extracellular water-soluble yellow pigment production were enhanced under H2O2-induced oxidative (HIO) conditions and were inhibited under dithiothreitol-induced reductive conditions. By optimizing the amount of H2O2 added and the timing of the addition, the yield of extracellular water-soluble yellow pigments significantly increased and reached a maximum of 209 AU, when 10 mM H2O2 was added on the 3rd day of fermentation with M. ruber CGMCC 10910. Under HIO conditions, the ratio of NADH/NAD+ was much lower than that in the control group, and the expression levels of relative pigment biosynthesis genes were up-regulated; moreover, the activity of glucose-6-phosphate dehydrogenase (G6PDH) was increased while 6-phosphofructokinase (PFK) activity was inhibited. Conclusions Oxidative conditions induced by H2O2 increased water-soluble yellow pigment accumulation via up-regulation of the expression levels of relative genes and by increasing the precursors of pigment biosynthesis through redirection of metabolic flux. In contrast, reductive conditions induced by dithiothreitol inhibited yellow pigment accumulation. This experiment provides a potential strategy for improving the production of Monascus yellow pigments.

Published in Microbial Cell Factories

ISSN: 1475-2859 (Online)
Publisher: BMC
Country of publisher: United Kingdom
LCC subjects: Science: Microbiology
Website: https://microbialcellfactories.biomedcentral.com/

About the journal

Abstract

Keywords