Screening data for the endocrine disrupting activities of 583 chemicals using the yeast two-hybrid assay
Fujio Shiraishi,
Ryo Kamata,
Masanori Terasaki,
Hidetaka Takigami,
Yoshitaka Imaizumi,
Mayuko Yagishita,
Daisuke Nakajima
Affiliations
Fujio Shiraishi
National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan
Ryo Kamata
Kitasato University, 23-35-1 Higashi, Towada, Aomori 034-8628, Japan
Masanori Terasaki
Iwate University, 3-18-8 Ueda, Morioka, Iwate 020-8550, Japan
Hidetaka Takigami
National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan
Yoshitaka Imaizumi
National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan
Mayuko Yagishita
National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan; Correspondence to: National Institute for Environmental Studies, Center for Health and Environmental Risk Research, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan.
Daisuke Nakajima
National Institute for Environmental Studies, 16-2 Onogawa, Tsukuba, Ibaraki 305-8506, Japan
We screened 583 chemicals for receptor binding activity to the human estrogen receptor (hER), the Japanese medaka estrogen receptor (medER), and the aryl hydrocarbon receptor (AhR) using the yeast two-hybrid assay. The substances tested included substances that could potentially be produced unintentionally by industrial processes, such as halogenated steroids and phenols. Antagonistic effects on hER and the androgen receptor were also screened. The test chemicals were selected for screening on the basis of chemical structure associated with possible estrogen receptor binding activity. The current study presents the report on the screening of 583 chemicals for different kinds of endocrine disrupting activity.
