An integrative exploration of loquat leaf total sesquiterpene glycosides in treating insulin-resistant mice by serum and urine untargeted metabolomics analysis
Yanan Gai,
Jiawei Li,
Tunyu Jian,
Xiaoqin Ding,
Han Lyu,
Yan Liu,
Jing Li,
Bingru Ren,
Jian Chen,
Weilin Li
Affiliations
Yanan Gai
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
Jiawei Li
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
Tunyu Jian
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
Xiaoqin Ding
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
Han Lyu
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
Yan Liu
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
Jing Li
Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China
Bingru Ren
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China
Jian Chen
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China; Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China; Corresponding author.
Weilin Li
Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China; Department of Food Science and Technology, College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China
Loquat leaf is approved to be beneficial in the treatment of diabetes. Total sesquiterpene glycosides (TSG), a major chemical component cluster, has potential ability to improve insulin-resistant diabetes syndrome. Its therapeutic mechanism using metabolomics in vivo is worth to be investigated. This study aimed to reveal the underlying therapeutic mechanism of TSG on insulin-resistant mice by untargeted metabolomics, and to explore the lipid metabolism differences in vivo. High-fat diet was used to induce insulin-resistant mice model. Biochemical indicators were applied to evaluate the model validity and related treatment effect. Ultra-performance liquid chromatography quadrupole-time-of-flight mass spectrometry was utilized to accomplish serum and urine untargeted metabolomics. Oral administration of TSG had a therapeutic effect on high-fat diet induced insulin-resistant mice. Four hundred forty-two metabolites in serum and 1732 metabolites in urine were annotated. Principal component analysis screened 324 differential metabolic signatures in serum sample and 1408 in urine sample. The pathway mainly involved purine metabolism and biosynthesis of unsaturated fatty acids. Lipidomic analysis of urine and serum confirmed that most lipid metabolites were fatty acyls, sterol lipids and polyketides.
