Wnt/β-Catenin Signaling Defines Organizing Centers that Orchestrate Growth and Differentiation of the Regenerating Zebrafish Caudal Fin

Cell Reports. 2014;6(3):467-481 DOI 10.1016/j.celrep.2013.12.036

 

Journal Homepage

Journal Title: Cell Reports

ISSN: 2211-1247 (Online)

Publisher: Elsevier

LCC Subject Category: Science: Biology (General)

Country of publisher: Netherlands

Language of fulltext: English

Full-text formats available: PDF, HTML

 

AUTHORS

Daniel Wehner (Institute for Biochemistry and Molecular Biology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany)
Wiebke Cizelsky (Institute for Biochemistry and Molecular Biology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany)
Mohankrishna Dalvoy Vasudevaro (Institute for Biochemistry and Molecular Biology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany)
Günes Özhan (Biotechnology Center, Technische Universität Dresden, Tatzberg 47-49, 01307 Dresden, Germany)
Christa Haase (Biotechnology Center, Technische Universität Dresden, Tatzberg 47-49, 01307 Dresden, Germany)
Birgit Kagermeier-Schenk (Biotechnology Center, Technische Universität Dresden, Tatzberg 47-49, 01307 Dresden, Germany)
Alexander Röder (Biotechnology Center, Technische Universität Dresden, Tatzberg 47-49, 01307 Dresden, Germany)
Richard I. Dorsky (Department of Neurobiology and Anatomy, University of Utah, Salt Lake City, UT 84112, USA)
Enrico Moro (Department of Molecular Medicine, University of Padova, 35121 Padova, Italy)
Francesco Argenton (Department of Biology, University of Padova, 35121 Padova, Italy)
Michael Kühl (Institute for Biochemistry and Molecular Biology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany)
Gilbert Weidinger (Institute for Biochemistry and Molecular Biology, Ulm University, Albert-Einstein-Allee 11, 89081 Ulm, Germany)

EDITORIAL INFORMATION

Blind peer review

Editorial Board

Instructions for authors

Time From Submission to Publication: 31 weeks

 

Abstract | Full Text

Zebrafish regenerate their fins via the formation of a population of progenitor cells, the blastema. Wnt/β-catenin signaling is essential for blastemal cell proliferation and patterning of the overlying epidermis. Yet, we find that β-catenin signaling is neither active in the epidermis nor the majority of the proliferative blastemal cells. Rather, tissue-specific pathway interference indicates that Wnt signaling in the nonproliferative distal blastema is required for cell proliferation in the proximal blastema, and signaling in cells lining the osteoblasts directs osteoblast differentiation. Thus, Wnt signaling regulates epidermal patterning, blastemal cell proliferation, and osteoblast maturation indirectly via secondary signals. Gene expression profiling, chromatin immunoprecipitation, and functional rescue experiments suggest that Wnt/β-catenin signaling acts through Fgf and Bmp signaling to control epidermal patterning, whereas retinoic acid and Hedgehog signals mediate its effects on blastemal cell proliferation. We propose that Wnt signaling orchestrates fin regeneration by defining organizing centers that instruct cellular behaviors of adjacent tissues.