The antibodies against the A137R protein drive antibody-dependent enhancement of African swine fever virus infection in porcine alveolar macrophages

Xiaoke Yang; Encheng Sun; Huanjie Zhai; Tao Wang; Shida Wang; Yuxuan Gao; Qinghe Hou; Xiangyu Guan; Shuwen Li; Lian-Feng Li; Hongxia Wu; Yuzi Luo; Su Li; Yuan Sun; Dongming Zhao; Yongfeng Li; Hua-Ji Qiu

doi:10.1080/22221751.2024.2377599

Emerging Microbes and Infections (Dec 2024)

The antibodies against the A137R protein drive antibody-dependent enhancement of African swine fever virus infection in porcine alveolar macrophages

Xiaoke Yang,
Encheng Sun,
Huanjie Zhai,
Tao Wang,
Shida Wang,
Yuxuan Gao,
Qinghe Hou,
Xiangyu Guan,
Shuwen Li,
Lian-Feng Li,
Hongxia Wu,
Yuzi Luo,
Su Li,
Yuan Sun,
Dongming Zhao,
Yongfeng Li,
Hua-Ji Qiu

Affiliations

Xiaoke Yang: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Encheng Sun: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Huanjie Zhai: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Tao Wang: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Shida Wang: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Yuxuan Gao: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Qinghe Hou: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Xiangyu Guan: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Shuwen Li: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Lian-Feng Li: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Hongxia Wu: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Yuzi Luo: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Su Li: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Yuan Sun: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Dongming Zhao: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Yongfeng Li: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China
Hua-Ji Qiu: State Key Laboratory for Animal Disease Control and Prevention, National High Containment Facilities for Animal Diseases Control and Prevention, Harbin Veterinary Research Institute, CAAS, Harbin, People’s Republic of China

DOI: https://doi.org/10.1080/22221751.2024.2377599
Journal volume & issue: Vol. 13, no. 1

Abstract

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African swine fever virus (ASFV) is the causative agent of African swine fever (ASF), a highly contagious disease that can kill up to 100% of domestic pigs and wild boars. It has been shown that the pigs inoculated with some ASF vaccine candidates display more severe clinical signs and die earlier than do pigs not immunized. We hypothesize that antibody-dependent enhancement (ADE) of ASFV infection may be caused by the presence of some unidentified antibodies. In this study, we found that the ASFV-encoded structural protein A137R (pA137R) can be recognized by the anti-ASFV positive sera, indicating that the anti-pA137R antibodies are induced in the ASFV-infected pigs. Interestingly, our results demonstrated that the anti-pA137R antibodies produced in rabbits or pigs enhanced viral replication of different ASFV strains in primary porcine alveolar macrophages (PAMs), the target cells of ASFV. Mechanistic investigations revealed that anti-pA137R antibodies were able to promote the attachment of ASFV to PAMs and two types of Fc gamma receptors (FcγRs), FcγRII and FcγRIII, mediated the ADE of ASFV infection. Taken together, anti-pA137R antibodies are able to drive ASFV ADE in PAMs. These findings shed new light on the roles of anti-ASFV antibodies and have implications for the pathophysiology of the disease and the development of ASF vaccines.

Published in Emerging Microbes and Infections

ISSN: 2222-1751 (Online)
Publisher: Taylor & Francis Group
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases; Science: Microbiology
Website: https://www.tandfonline.com/journals/temi

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