Photometric adaptation of Dole's microdetermination of free fatty acids

Abstract

The method is based on the measurement of color changes in phenol red barbital buffer in heptane–ethanol. It has been applied to human serum and to incubation media containing fatty acids liberated from rat epididymal adipose tissue. The values obtained were found to agree with those using the titrimetric method, within the limit of experimental error.

Keywords

• free fatty acids
• determination
• color-imetry
• serum
• incubation media