Mapeamento das interações proteína-ligante através de técnicas de RMN de ¹H utilizando detecção do ligante Screening protein-ligand interactions using ¹H NMR techniques for detecting the ligand

Abstract

NMR is a valuable screening tool for the binding of ligands to proteins providing structural information on both protein and ligands and is thus largely applied to drug-discovery. Among the recent NMR techniques to probe weak binding protein-ligand complexes we have critically evaluated the advantages and disadvantages of STD (Saturation Transfer Difference), WaterLOGSY (Water Ligand Observation with Gradient Spectroscopy), NOE pumping and DOSY-NOESY (Diffusion-Ordered NOESY) using a mixture of BSA (bovine serum albumin) plus salicylic acid, caffeine, citric acid, adipic acid and D-glucose.

Keywords

• NMR
• protein-ligand interactions
• bovine serum albumin