Antibacterial Calcium Phosphate Coatings for Biomedical Applications Fabricated via Micro-Arc Oxidation
Anna I. Kozelskaya,
Ksenia N. Verzunova,
Igor O. Akimchenko,
Johannes Frueh,
Vsevolod I. Petrov,
Galina B. Slepchenko,
Olga V. Bakina,
Marat I. Lerner,
Leonid K. Brizhan,
Denis V. Davydov,
Artur A. Kerimov,
Elena G. Cherempey,
Sergey E. Krylov,
Sven Rutkowski,
Sergei I. Tverdokhlebov
Affiliations
Anna I. Kozelskaya
Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia
Ksenia N. Verzunova
Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia
Igor O. Akimchenko
Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia
Johannes Frueh
Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia
Vsevolod I. Petrov
Tomsk Scientific Center of the Siberian Branch of the Russian Academy of Sciences, 10/4, Akademicheskii Prospekt, 634055 Tomsk, Russia
Galina B. Slepchenko
Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia
Olga V. Bakina
Institute of Strength Physics and Materials Science of the Siberian Branch of the Russian Academy of Sciences, 2/4, Akademicheskii Prospekt, 634055 Tomsk, Russia
Marat I. Lerner
Institute of Strength Physics and Materials Science of the Siberian Branch of the Russian Academy of Sciences, 2/4, Akademicheskii Prospekt, 634055 Tomsk, Russia
Leonid K. Brizhan
Federal State Budgetary Institution «Main Military Clinical Hospital Named after Academician N.N. Burdenko» of the Ministry of defense of the Russian Federation», 3 Gospitalnaya Square, 105299 Moscow, Russia
Denis V. Davydov
Federal State Budgetary Institution «Main Military Clinical Hospital Named after Academician N.N. Burdenko» of the Ministry of defense of the Russian Federation», 3 Gospitalnaya Square, 105299 Moscow, Russia
Artur A. Kerimov
Federal State Budgetary Institution «Main Military Clinical Hospital Named after Academician N.N. Burdenko» of the Ministry of defense of the Russian Federation», 3 Gospitalnaya Square, 105299 Moscow, Russia
Elena G. Cherempey
UMH LLC, 48 Govorova Street, Apt. 191, 634050 Tomsk, Russia
Sergey E. Krylov
BITECA LLC, 9 Zapadnaya Street, Building 10, 143002 Odintsovo, Russia
Sven Rutkowski
Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia
Sergei I. Tverdokhlebov
Weinberg Research Center, School of Nuclear Science &Engineering, Tomsk Polytechnic University, 30, Lenin Avenue, 634050 Tomsk, Russia
A promising method for improving the functional properties of calcium-phosphate coatings is the incorporation of various antibacterial additives into their structure. The microbial contamination of a superficial wound is inevitable, even if the rules of asepsis and antisepsis are optimally applied. One of the main problems is that bacteria often become resistant to antibiotics over time. However, this does not apply to certain elements, chemical compounds and drugs with antimicrobial properties. In this study, the fabrication and properties of zinc-containing calcium-phosphate coatings that were formed via micro-arc oxidation from three different electrolyte solutions are investigated. The first electrolyte is based on calcium oxide, the second on hydroxyapatite and the third on calcium acetate. By adding zinc oxide to the three electrolyte solutions, antibacterial properties of the coatings are achieved. Although the same amount of zinc oxide has been added to each electrolyte solution, the zinc concentration in the coatings obtained vary greatly. Furthermore, this study investigates the morphology, structure and chemical composition of the coatings. The antibacterial properties of the zinc-containing coatings were tested toward three strains of bacteria—Staphylococcus aureus, methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa. Coatings of calcium acetate and zinc oxide contained the highest amount of zinc and displayed the highest zinc release. Moreover, coatings containing hydroxyapatite and zinc oxide show the highest antibacterial activity toward Pseudomonas aeruginosa, and coatings containing calcium acetate and zinc oxide show the highest antibacterial activities toward Staphylococcus aureus and methicillin-resistant Staphylococcus aureus.
