Nature Communications (Aug 2022)
Muconic acid production from glucose and xylose in Pseudomonas putida via evolution and metabolic engineering
- Chen Ling,
- George L. Peabody,
- Davinia Salvachúa,
- Young-Mo Kim,
- Colin M. Kneucker,
- Christopher H. Calvey,
- Michela A. Monninger,
- Nathalie Munoz Munoz,
- Brenton C. Poirier,
- Kelsey J. Ramirez,
- Peter C. St. John,
- Sean P. Woodworth,
- Jon K. Magnuson,
- Kristin E. Burnum-Johnson,
- Adam M. Guss,
- Christopher W. Johnson,
- Gregg T. Beckham
Affiliations
- Chen Ling
- Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory
- George L. Peabody
- Agile BioFoundry
- Davinia Salvachúa
- Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory
- Young-Mo Kim
- Agile BioFoundry
- Colin M. Kneucker
- Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory
- Christopher H. Calvey
- Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory
- Michela A. Monninger
- Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory
- Nathalie Munoz Munoz
- Agile BioFoundry
- Brenton C. Poirier
- Agile BioFoundry
- Kelsey J. Ramirez
- Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory
- Peter C. St. John
- Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory
- Sean P. Woodworth
- Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory
- Jon K. Magnuson
- Agile BioFoundry
- Kristin E. Burnum-Johnson
- Agile BioFoundry
- Adam M. Guss
- Agile BioFoundry
- Christopher W. Johnson
- Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory
- Gregg T. Beckham
- Renewable Resources and Enabling Sciences Center, National Renewable Energy Laboratory
- DOI
- https://doi.org/10.1038/s41467-022-32296-y
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 14
Abstract
Muconic acid is a platform chemical with wide industrial applicability. Here, the authors report efficient muconate production from glucose and xylose by engineered Pseudomonas putida strain using adaptive laboratory evolution, metabolic modeling, and rational strain engineering strategies.
