Altered Response to Total Body Irradiation of C57BL/6-Tg (CAG-EGFP) Mice
Cuihua Liu,
Kaoru Tanaka,
Takanori Katsube,
Guillaume Varès,
Kouichi Maruyama,
Yasuharu Ninomiya,
Zeenath Fardous,
Chao Sun,
Akira Fujimori,
Stéphanie G. Moreno,
Mitsuru Nenoi,
Bing Wang
Affiliations
Cuihua Liu
, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan
Kaoru Tanaka
, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan
Takanori Katsube
, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan
Guillaume Varès
Cell Signal Unit, , Okinawa, Japan
Kouichi Maruyama
, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan
Yasuharu Ninomiya
, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan
Zeenath Fardous
Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Bangladesh Atomic Energy Commission, People’s Republic of Bangladesh
Chao Sun
, Lanzhou, People’s Republic of China
Akira Fujimori
, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan
Stéphanie G. Moreno
LRTS—François Jacob Institute of Biology, Fundamental Research Division, Atomic Energy and Alternative Energies Commission, Inserm, Fontenay-aux-Roses Cedex, France
Mitsuru Nenoi
Department of Safety Administration, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan
Bing Wang
, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan
Application of green fluorescent protein (GFP) in a variety of biosystems as a unique bioindicator or biomarker has revolutionized biological research and made groundbreaking achievements, while increasing evidence has shown alterations in biological properties and physiological functions of the cells and animals overexpressing transgenic GFP. In this work, response to total body irradiation (TBI) was comparatively studied in GFP transgenic C57BL/6-Tg (CAG-EGFP) mice and C57BL/6 N wild type mice. It was demonstrated that GFP transgenic mice were more sensitive to radiation-induced bone marrow death, and no adaptive response could be induced. In the nucleated bone marrow cells of GFP transgenic mice exposed to a middle dose, there was a significant increase in both the percentage of cells expressing pro-apoptotic gene Bax and apoptotic cell death. While in wild type cells, lower expression of pro-apoptotic gene Bax and higher expression of anti-apoptotic gene Bcl-2, and significant lower induction of apoptosis were observed compared to GFP transgenic cells. Results suggest that presence of GFP could alter response to TBI at whole body, cellular and molecular levels in mice. These findings indicate that there could be a major influence on the interpretation of the results obtained in GFP transgenic mice.
