Targeted HPTLC Profile, Quantification of Flavonoids and Phenolic Acids, and Antimicrobial Activity of <i>Dodonaea angustifolia</i> (L.f.) Leaves and Flowers
Fekade Beshah Tessema,
Yilma Hunde Gonfa,
Tilahun Belayneh Asfaw,
Mesfin Getachew Tadesse,
Tigist Getachew Tadesse,
Archana Bachheti,
Mohammed O. Alshaharni,
Pankaj Kumar,
Vinod Kumar,
Ivan Širić,
Sami Abou Fayssal,
Kundan Kumar Chaubey,
Rakesh Kumar Bachheti
Affiliations
Fekade Beshah Tessema
Department of Industrial Chemistry, Addis Ababa Science and Technology University, Addis Ababa P.O. Box 16417, Ethiopia
Yilma Hunde Gonfa
Department of Industrial Chemistry, Addis Ababa Science and Technology University, Addis Ababa P.O. Box 16417, Ethiopia
Tilahun Belayneh Asfaw
Department of Industrial Chemistry, Addis Ababa Science and Technology University, Addis Ababa P.O. Box 16417, Ethiopia
Mesfin Getachew Tadesse
Department of Industrial Chemistry, Addis Ababa Science and Technology University, Addis Ababa P.O. Box 16417, Ethiopia
Tigist Getachew Tadesse
Bio and Emerging Technology Institute, Health Biotechnology Directorate, Addis Ababa P.O. Box 5954, Ethiopia
Archana Bachheti
Department of Environment Science, Graphic Era (Deemed to be University), Dehradun 248002, India
Mohammed O. Alshaharni
Department of Biology, College of Science, King Khalid University, Abha 61321, Saudi Arabia
Pankaj Kumar
Agro-Ecology and Pollution Research Laboratory, Department of Zoology and Environmental Science, Gurukula Kangri (Deemed to Be University), Haridwar 249404, India
Vinod Kumar
Agro-Ecology and Pollution Research Laboratory, Department of Zoology and Environmental Science, Gurukula Kangri (Deemed to Be University), Haridwar 249404, India
Ivan Širić
University of Zagreb, Faculty of Agriculture, Svetosimunska 25, 10000 Zagreb, Croatia
Sami Abou Fayssal
Department of Agronomy, Faculty of Agronomy, University of Forestry, 10 Kliment Ohridski Blvd, 1797 Sofia, Bulgaria
Kundan Kumar Chaubey
Division of Research and Innovation, School of Applied and Life Sciences, Uttaranchal University, Arcadia Grant, P.O. Chandanwari, Prem Nagar, Dehradun 248007, India
Rakesh Kumar Bachheti
Department of Industrial Chemistry, Addis Ababa Science and Technology University, Addis Ababa P.O. Box 16417, Ethiopia
In East Africa, Dodonaea angustifolia (L.f.) is a well-known medicinal herb. Its leaf is primarily studied in light of its ethnobotanical use. In terms of phytochemistry and biological activity, its flower is not studied. In a prior study, our team looked into phytochemical screening, antioxidant activity, and total phenolic levels. This study aims to compare the profiles and biological activities of the leaf and flower samples of D. angustifolia and to present therapeutic alternatives. The leaf and flower sample powders were extracted with methanol using ultrasound-assisted extraction (UAE). HPTLC profile was obtained using CAMAG—HPTLC equipped with VisionCATS software. Antimicrobial agar well diffusion assay and minimum inhibition concentration (MIC) were determined. The leaf and flower extracts of D. angustifolia showed antibacterial activity with a MIC value of 20 µg/mL against Enterococcus faecalis and Listeria monocytogenes. Similarly, 40 µg/mL was found to be effective against Aspergillus flavus. D. angustifolia flower is a rich source of flavonoids and phenolic acids. Because of its antibacterial properties and profile, which are almost the same, the flower is emerging as a viable option for medicinal alternatives.
