Structure and mechanism of the two-component α-helical pore-forming toxin YaxAB

Bastian Bräuning; Eva Bertosin; Florian Praetorius; Christian Ihling; Alexandra Schatt; Agnes Adler; Klaus Richter; Andrea Sinz; Hendrik Dietz; Michael Groll

doi:10.1038/s41467-018-04139-2

Nature Communications (May 2018)

Structure and mechanism of the two-component α-helical pore-forming toxin YaxAB

Bastian Bräuning,
Eva Bertosin,
Florian Praetorius,
Christian Ihling,
Alexandra Schatt,
Agnes Adler,
Klaus Richter,
Andrea Sinz,
Hendrik Dietz,
Michael Groll

Affiliations

Bastian Bräuning: Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry, Chair of Biochemistry, Technische Universität München
Eva Bertosin: Center for Integrated Protein Science Munich (CIPSM), Department of Physics, Technische Universität München
Florian Praetorius: Center for Integrated Protein Science Munich (CIPSM), Department of Physics, Technische Universität München
Christian Ihling: Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther University Halle-Wittenberg
Alexandra Schatt: Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther University Halle-Wittenberg
Agnes Adler: Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry, Chair of Biochemistry, Technische Universität München
Klaus Richter: Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry, Chair of Biotechnology, Technische Universität München
Andrea Sinz: Department of Pharmaceutical Chemistry and Bioanalytics, Institute of Pharmacy, Martin-Luther University Halle-Wittenberg
Hendrik Dietz: Center for Integrated Protein Science Munich (CIPSM), Department of Physics, Technische Universität München
Michael Groll: Center for Integrated Protein Science Munich (CIPSM), Department of Chemistry, Chair of Biochemistry, Technische Universität München

DOI: https://doi.org/10.1038/s41467-018-04139-2
Journal volume & issue: Vol. 9, no. 1
pp. 1 – 14

Abstract

Read online

The Yersinia YaxAB system is a pore-forming toxin of so far unknown structure. Here authors present X-ray and cryo-EM to structures of individual subunits and of the YaxAB pore complex, and find that YaxA binds to membranes first and recruits YaxB for subsequent oligomerization.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

About the journal