Kidney epithelial cells are active mechano-biological fluid pumps

Mohammad Ikbal Choudhury; Yizeng Li; Panagiotis Mistriotis; Ana Carina N. Vasconcelos; Eryn E. Dixon; Jing Yang; Morgan Benson; Debonil Maity; Rebecca Walker; Leigha Martin; Fatima Koroma; Feng Qian; Konstantinos Konstantopoulos; Owen M. Woodward; Sean X. Sun

doi:10.1038/s41467-022-29988-w

Nature Communications (Apr 2022)

Kidney epithelial cells are active mechano-biological fluid pumps

Mohammad Ikbal Choudhury,
Yizeng Li,
Panagiotis Mistriotis,
Ana Carina N. Vasconcelos,
Eryn E. Dixon,
Jing Yang,
Morgan Benson,
Debonil Maity,
Rebecca Walker,
Leigha Martin,
Fatima Koroma,
Feng Qian,
Konstantinos Konstantopoulos,
Owen M. Woodward,
Sean X. Sun

Affiliations

Mohammad Ikbal Choudhury: Department of Mechanical Engineering, Johns Hopkins University
Yizeng Li: Department of Mechanical Engineering, Johns Hopkins University
Panagiotis Mistriotis: Department of Chemical and Biomolecular Engineering, Johns Hopkins University
Ana Carina N. Vasconcelos: Department of Mechanical Engineering, Johns Hopkins University
Eryn E. Dixon: Department of Biochemistry and Molecular Biology, Maryland PKD Research and Clinical Core Center, University of Maryland School of Medicine
Jing Yang: Department of Mechanical Engineering, Johns Hopkins University
Morgan Benson: Institute of NanoBioTechnology, Johns Hopkins University
Debonil Maity: Institute of NanoBioTechnology, Johns Hopkins University
Rebecca Walker: Department of Biochemistry and Molecular Biology, Maryland PKD Research and Clinical Core Center, University of Maryland School of Medicine
Leigha Martin: Department of Chemical and Biomolecular Engineering, Johns Hopkins University
Fatima Koroma: Department of Chemical and Biomolecular Engineering, Johns Hopkins University
Feng Qian: Department of Biochemistry and Molecular Biology, Maryland PKD Research and Clinical Core Center, University of Maryland School of Medicine
Konstantinos Konstantopoulos: Institute of NanoBioTechnology, Johns Hopkins University
Owen M. Woodward: Department of Physiology, Maryland PKD Research and Clinical Core Center, University of Maryland School of Medicine
Sean X. Sun: Department of Mechanical Engineering, Johns Hopkins University

DOI: https://doi.org/10.1038/s41467-022-29988-w
Journal volume & issue: Vol. 13, no. 1
pp. 1 – 13

Abstract

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How mechanical forces drive fluid transport in the kidney remains unclear. Here, the authors use a microfluidic platform to show that kidney epithelial cells generate hydraulic pressure gradients across the epithelium, and that the fluid flux is from apical to basal for normal cells, and inverted in autosomal dominant polycystic kidney disease cells.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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