Novel Optical Criteria and Mechanisms of Critical Decline in Liver Regenerative Potential
Svetlana Rodimova,
Vera Kozlova,
Nikolai Bobrov,
Dmitry Kozlov,
Artem Mozherov,
Vadim Elagin,
Ilya Shchechkin,
Dmitry Kuzmin,
Alena Gavrina,
Vladimir Zagainov,
Elena Zagaynova,
Daria Kuznetsova
Affiliations
Svetlana Rodimova
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, Russia
Vera Kozlova
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, Russia
Nikolai Bobrov
The Volga District Medical Centre of Federal Medical and Biological Agency, 14 Ilinskaya Str., 603000 Nizhny Novgorod, Russia
Dmitry Kozlov
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, Russia
Artem Mozherov
Laboratory of Omics and Regenerative Technologies, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8–2 Trubetskaya Str., 119991 Moscow, Russia
Vadim Elagin
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, Russia
Ilya Shchechkin
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, Russia
Dmitry Kuzmin
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, Russia
Alena Gavrina
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, Russia
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, Russia
Daria Kuznetsova
Institute of Experimental Oncology and Biomedical Technologies, Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Sq., 603000 Nizhny Novgorod, Russia
The most effective method of treating tumors localized in the liver remains resection. However, in the presence of concomitant pathology, the regenerative potential of the liver is significantly reduced. To date, there is insufficient fundamental data on the mechanisms responsible for the disruption of liver regeneration, and there is no effective method for assessing its regenerative potential. The most suitable model for these purposes is acute liver injury (ALI). Modern non-contrast methods of multiphoton microscopy with second harmonic generation and fluorescence lifetime imaging microscopy (FLIM) modes enable intravital evaluation of the metabolic status of the hepatocytes; therefore, this expands the possibilities for studying the processes occurring in cells during regeneration in the context of any pathologies.
