Cancer Management and Research (Oct 2019)
Upregulation Of circMMP9 Promotes Osteosarcoma Progression Via Targeting miR-1265/CHI3L1 Axis
Abstract
Guangjie Pan,1 Ting Hu,1 Xuewu Chen,1 Chao Zhang2 1Department of Orthopedics, Wenzhou Central Hospital, Wenzhou 325000, People’s Republic of China; 2Department of Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325000, People’s Republic of ChinaCorrespondence: Chao ZhangDepartment of Orthopedics, The First Affiliated Hospital of Wenzhou Medical University, Shangcai Village, Ouhai District, Wenzhou 325000, People’s Republic of ChinaTel +86 15958710270Email [email protected]: Osteosarcoma (OS) is a very aggressive cancer. Nevertheless, how circular RNA (circRNA) contributes to OS progression remains unclear. Here, we aimed to research the functions of circMMP9 in OS progression.Methods: Gene expression was determined via qRT-PCR. siRNA was used to knock down circMMP9. Proliferation was analyzed using CCK8 and colony formation assays. Migration and invasion were measured using Transwell assay.Results: circMMP9 was overexpressed in cancer tissues. Overexpressed circMMP9 was correlated with advanced tumor stage and predicted poor prognosis. circMMP9 knockdown exhibited a tumor-suppressive phenotype via suppressing proliferation, migration and invasion. Besides, decreased circMMP9 level promoted OS cellular apoptosis. Mechanistically, circMMP9 was shown to be located in the cytoplasm and sponge miR-1265. Furthermore, miR-1265 directly targeted CHI3L1. CHI3L1 levels were modulated by circMMP9/miR-1265 axis. Rescue experiments indicated that circMMP9 contributes to OS development through the miR-1265/CHI3L1 pathway.Conclusion: Our findings provide a novel insight about how circRNA regulates OS progression.Keywords: circMMP9, miR-1265, CHI3L1, osteosarcoma, progression
