Frontiers in Pharmacology (Feb 2022)
Protective Effect of Isoorientin on Oleic Acid-Induced Oxidative Damage and Steatosis in Rat Liver Cells
- Tongwang Luo,
- Tongwang Luo,
- Tongwang Luo,
- Tongwang Luo,
- Tongwang Luo,
- Sheng Jiang,
- Sheng Jiang,
- Sheng Jiang,
- Sheng Jiang,
- Sheng Jiang,
- Bin Zhou,
- Bin Zhou,
- Bin Zhou,
- Bin Zhou,
- Bin Zhou,
- Quanjiang Song,
- Quanjiang Song,
- Quanjiang Song,
- Quanjiang Song,
- Quanjiang Song,
- Jing Du,
- Jing Du,
- Jing Du,
- Jing Du,
- Jing Du,
- Ping Liu,
- Ping Liu,
- Ping Liu,
- Ping Liu,
- Ping Liu,
- Xiaodu Wang,
- Xiaodu Wang,
- Xiaodu Wang,
- Xiaodu Wang,
- Xiaodu Wang,
- Houhui Song,
- Houhui Song,
- Houhui Song,
- Houhui Song,
- Houhui Song,
- Chunyan Shao,
- Chunyan Shao,
- Chunyan Shao,
- Chunyan Shao,
- Chunyan Shao
Affiliations
- Tongwang Luo
- Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China
- Tongwang Luo
- Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China
- Tongwang Luo
- Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China
- Tongwang Luo
- China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China
- Tongwang Luo
- College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China
- Sheng Jiang
- Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China
- Sheng Jiang
- Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China
- Sheng Jiang
- Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China
- Sheng Jiang
- China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China
- Sheng Jiang
- College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China
- Bin Zhou
- Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China
- Bin Zhou
- Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China
- Bin Zhou
- Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China
- Bin Zhou
- China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China
- Bin Zhou
- College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China
- Quanjiang Song
- Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China
- Quanjiang Song
- Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China
- Quanjiang Song
- Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China
- Quanjiang Song
- China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China
- Quanjiang Song
- College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China
- Jing Du
- Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China
- Jing Du
- Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China
- Jing Du
- Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China
- Jing Du
- China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China
- Jing Du
- College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China
- Ping Liu
- Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China
- Ping Liu
- Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China
- Ping Liu
- Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China
- Ping Liu
- China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China
- Ping Liu
- College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China
- Xiaodu Wang
- Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China
- Xiaodu Wang
- Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China
- Xiaodu Wang
- Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China
- Xiaodu Wang
- China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China
- Xiaodu Wang
- College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China
- Houhui Song
- Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China
- Houhui Song
- Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China
- Houhui Song
- Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China
- Houhui Song
- China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China
- Houhui Song
- College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China
- Chunyan Shao
- Key Laboratory of Applied Technology on Green-Eco-Healthy Animal Husbandry of Zhejiang Province, Hangzhou, China
- Chunyan Shao
- Zhejiang Provincial Engineering Laboratory for Animal Health Inspection and Internet Technology, Hangzhou, China
- Chunyan Shao
- Zhejiang International Science and Technology Cooperation Base for Veterinary Medicine and Health Management, Hangzhou, China
- Chunyan Shao
- China-Australia Joint Laboratory for Animal Health Big Data Analytics, Hangzhou, China
- Chunyan Shao
- College of Animal Science and Technology, College of Veterinary Medicine, Zhejiang A&F University, Hangzhou, China
- DOI
- https://doi.org/10.3389/fphar.2022.818159
- Journal volume & issue
-
Vol. 13
Abstract
The harm of nonalcoholic fatty liver disease to human health is increasing, which calls for urgent prevention and treatment of the disease. Isoorientin is an effective ingredient of Chinese herbal medicine with anti-inflammatory and antioxidant effects. However, the effect of isoorientin in nonalcoholic fatty liver disease is still unclear. In this study, combined in vivo and in vitro experiments, through pathological observation, flow cytometry, immunofluorescence and western blot analysis to explore the role of isoorientin in steatosis and reveal its molecular mechanism. The results demonstrated that oleic acid treatment significantly increased the content of ROS and lipid droplets in rat hepatocytes, and promoted the expression of γH2AX, HO-1, PPARγ, SREBP-1c, FAS. The ROS content in the cells of co-treated with isoorientin and oleic acid was significantly reduced compared to the oleic acid group, and the expression of γH2AX, HO-1, PPARγ, SREBP-1c, FAS, and the nuclear translocation of NF-κB p65 were also significantly inhibited. Our data showed that oleic acid induce oxidative damage and steatosis in hepatocytes both in vitro and in vivo, and activate the PPARγ/NF-κB p65 signal pathway. Moreover, isoorientin can significantly reduce oleic acid -induced oxidative damage and steatosis by regulating the PPARγ/NF-kB p65 signal pathway.
Keywords
