miRNA Stability in Frozen Plasma Samples

Francesca Balzano; Marta Deiana; Silvia Dei Giudici; Annalisa Oggiano; Angela Baralla; Sara Pasella; Andrea Mannu; Mario Pescatori; Baingio Porcu; Giuseppe Fanciulli; Angelo Zinellu; Ciriaco Carru; Luca Deiana

doi:10.3390/molecules201019030

Molecules (Oct 2015)

miRNA Stability in Frozen Plasma Samples

Francesca Balzano,
Marta Deiana,
Silvia Dei Giudici,
Annalisa Oggiano,
Angela Baralla,
Sara Pasella,
Andrea Mannu,
Mario Pescatori,
Baingio Porcu,
Giuseppe Fanciulli,
Angelo Zinellu,
Ciriaco Carru,
Luca Deiana

Affiliations

Francesca Balzano: Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy
Marta Deiana: Associazione “L’Isola dei Centenari”, Via Milano 4, Sassari 07100, Italy
Silvia Dei Giudici: Istituto Zooprofilattico Sperimentale della Sardegna, Via Vienna 2, Sassari 07100, Italy
Annalisa Oggiano: Istituto Zooprofilattico Sperimentale della Sardegna, Via Vienna 2, Sassari 07100, Italy
Angela Baralla: Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy
Sara Pasella: Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy
Andrea Mannu: Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy
Mario Pescatori: Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy
Baingio Porcu: Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy
Giuseppe Fanciulli: Department of Clinical and Experimental Medicine, University of Sassari, vl. San Pietro 8, Sassari 07100, Italy
Angelo Zinellu: Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy
Ciriaco Carru: Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy
Luca Deiana: Department of Biomedical Sciences, University of Sassari, vl. San Pietro 43b, Sassari 07100, Italy

DOI: https://doi.org/10.3390/molecules201019030
Journal volume & issue: Vol. 20, no. 10
pp. 19030 – 19040

Abstract

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MicroRNAs (miRNAs) represent a family of small non-coding ribonucleic acids that post-transcriptionally inhibits the expression of their target messenger RNAs (mRNAs), thereby acting as general gene repressors. In this study we examined the relative quantity and stability of miRNA subjected to a long period of freezing; we compared the stability of eight miRNAs in the plasma of five human healthy controls before freezing and after six and 12 months of storage at −80 °C. In addition, we examined the plasma frozen for 14 years and the amount of miRNA still available. Using a Life Technologies protocol to amplify and quantify plasma miRNAs from EDTA (Ethylene Diamine Tetraacetic Acid)-treated blood, we analyzed the stability of eight miRNAs, (miR-125b-5p, miR-425-5p, miR-200b-5p, miR-200c-3p, miR-579-3p, miR-212-3p, miR-126-3p, and miR-21-5p). The miRNAs analyzed showed a high stability and long frozen half-life.

Published in Molecules

ISSN: 1420-3049 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Chemistry: Organic chemistry
Website: http://www.mdpi.com/journal/molecules

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