Nature Communications (Sep 2022)

Structure of the PAPP-ABP5 complex reveals mechanism of substrate recognition

  • Russell A. Judge,
  • Janani Sridar,
  • Kathryn Tunyasunvunakool,
  • Rinku Jain,
  • John C. K. Wang,
  • Christna Ouch,
  • Jun Xu,
  • Amirhossein Mafi,
  • Aaron H. Nile,
  • Clint Remarcik,
  • Corey L. Smith,
  • Crystal Ghosh,
  • Chen Xu,
  • Vincent Stoll,
  • John Jumper,
  • Amoolya H. Singh,
  • Dan Eaton,
  • Qi Hao

DOI
https://doi.org/10.1038/s41467-022-33175-2
Journal volume & issue
Vol. 13, no. 1
pp. 1 – 13

Abstract

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PAPP-A substrate selectivity underlies the tight regulation of IGF signaling. Here, the authors report cryo-EM structures of dimeric PAPP-A in its substrate-free form and in complex with a peptide substrate, which combined with biochemical assays provide a mechanism for PAPP-A substrate binding and selectivity.