Frontiers in Microbiology (May 2022)
Identification of a Novel Astrovirus in Pinnipeds
- Peijun Zhang,
- Haoxiang Su,
- Ruoyan Peng,
- Ruoyan Peng,
- Ruoyan Peng,
- Jasper Fuk-Woo Chan,
- Jasper Fuk-Woo Chan,
- Shijie Bai,
- Gaoyu Wang,
- Gaoyu Wang,
- Gaoyu Wang,
- Yi Huang,
- Yi Huang,
- Yi Huang,
- Xiaoyuan Hu,
- Xiaoyuan Hu,
- Xiaoyuan Hu,
- Jun Luo,
- Sisi Liu,
- Youyou Li,
- Youyou Li,
- Youyou Li,
- Liying Xue,
- Liying Xue,
- Liying Xue,
- Fan Yang,
- Mingming Zhao,
- Yun Zhang,
- Yun Zhang,
- Yun Zhang,
- Chuanning Tang,
- Chuanning Tang,
- Chuanning Tang,
- Shu Shen,
- Xiuji Cui,
- Xiuji Cui,
- Xiuji Cui,
- Lina Niu,
- Lina Niu,
- Lina Niu,
- Gang Lu,
- Gang Lu,
- Gang Lu,
- Kwok-Yung Yuen,
- Kwok-Yung Yuen,
- Fei Deng,
- Weijia Zhang,
- Feifei Yin,
- Feifei Yin,
- Feifei Yin,
- Jiang Du,
- Jiang Du,
- Jiang Du
Affiliations
- Peijun Zhang
- Marine Mammal and Marine Bioacoustics Laboratory, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Haoxiang Su
- NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
- Ruoyan Peng
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Ruoyan Peng
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Ruoyan Peng
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Jasper Fuk-Woo Chan
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Jasper Fuk-Woo Chan
- State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
- Shijie Bai
- Laboratory of Marine Viruses and Molecular Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Gaoyu Wang
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Gaoyu Wang
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Gaoyu Wang
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Yi Huang
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Yi Huang
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Yi Huang
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Xiaoyuan Hu
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Xiaoyuan Hu
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Xiaoyuan Hu
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Jun Luo
- Dalian Sunasia Tourism Holding Co., Ltd., Dalian, China
- Sisi Liu
- 0Qingdao Polar Haichang Ocean Park, Qingdao, China
- Youyou Li
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Youyou Li
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Youyou Li
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Liying Xue
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Liying Xue
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Liying Xue
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Fan Yang
- NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
- Mingming Zhao
- 1TCM School of Hainan Medical University, Haikou, China
- Yun Zhang
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Yun Zhang
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Yun Zhang
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Chuanning Tang
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Chuanning Tang
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Chuanning Tang
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Shu Shen
- 2State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
- Xiuji Cui
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Xiuji Cui
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Xiuji Cui
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Lina Niu
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Lina Niu
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Lina Niu
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Gang Lu
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Gang Lu
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Gang Lu
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Kwok-Yung Yuen
- Hainan Medical University-The University of Hong Kong Joint Laboratory of Tropical Infectious Diseases, Hainan Medical University, Haikou, China
- Kwok-Yung Yuen
- State Key Laboratory of Emerging Infectious Diseases, The University of Hong Kong, Pokfulam, Hong Kong SAR, China
- Fei Deng
- 2State Key Laboratory of Virology and National Virus Resource Center, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan, China
- Weijia Zhang
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Feifei Yin
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Feifei Yin
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- Feifei Yin
- Department of Pathogen Biology, Hainan Medical University, Haikou, China
- Jiang Du
- NHC Key Laboratory of Systems Biology of Pathogens, Institute of Pathogen Biology, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China
- Jiang Du
- Key Laboratory of Tropical Translational Medicine of Ministry of Education, Hainan Medical University, Haikou, China
- Jiang Du
- Laboratory of Deep Sea Microbial Cell Biology, Institute of Deep-Sea Science and Engineering, Chinese Academy of Sciences, Sanya, China
- DOI
- https://doi.org/10.3389/fmicb.2022.845601
- Journal volume & issue
-
Vol. 13
Abstract
Astroviruses infect human and animals and cause diarrhea, fever, and vomiting. In severe cases, these infections may be fatal in infants and juvenile animals. Previous evidence showed that humans in contact with infected animals can develop serological responses to astroviruses. Mamastrovirus 11 is a species of Mamastrovirus and was first reported in 2018. It was detected in the fecal samples of a California sea lion. The genome sequence of its capsid protein (CP) was submitted to GenBank. However, the genome sequence of its non-structural protein region was not elucidated. In the present study, we characterized the genome sequences of the novel astroviruses AstroV-HMU-1 and AstroV-like-HMU-2. These were obtained from California sea lions (Zalophus californianus) and walruses (Odobenus rosmarus) presenting with loose stools. A phylogenetic analysis revealed that the CP of AstroV-HMU-1 closely clustered with Mamastrovirus 11 while its RNA-dependent RNA polymerase (RdRp) and serine protease (SP) were closely related to the mink astrovirus in the genus Mamastrovirus. The genome of AstroV-HMU-1 provided basic information regarding the NS protein regions of Mamastrovirus 11. Recombination analyses showed that the genomes of Z. californianus AstroV-HMU-1, VA2/human and the mink astrovirus may have recombined long ago. The NS of AstroV-like-HMU-2 segregated from the Astroviridae in the deep root of the phylogenetic tree and exhibited 36% amino acid identity with other mamastroviruses. Thus, AstroV-like-HMU-2 was proposed as a member of a new genus in the unclassified Astroviridae. The present study suggested that that the loose stools of pinnipeds may be the result of occasional infection by this novel astrovirus. This discovery provides a scientific basis for future investigations into other animal-borne infectious diseases.
Keywords
