Preparation and Integration of a Multi-Wavelength LED Matrix for Testing Light Cell Interaction in a Novel Lens Less Optical Microscope
Rudolf Heer,
Sigurd Krieger,
Silvana Geleff,
Jörg Schotter,
Wenze Wu,
Gregor Scholz,
Hutomo Suryo Wasisto
Affiliations
Rudolf Heer
AIT Austrian Institute of Technology GmbH, Giefinggasse 4, 1210 Vienna, Austria
Sigurd Krieger
Department of Pathology, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria
Silvana Geleff
Department of Pathology, Medical University of Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria
Jörg Schotter
AIT Austrian Institute of Technology GmbH, Giefinggasse 4, 1210 Vienna, Austria
Wenze Wu
Institute of Semiconductor Technology (IHT) and Laboratory for Emerging Nanometrology (LENA), Technische Universität Braunschweig, 38106 Braunschweig, Germany
Gregor Scholz
Institute of Semiconductor Technology (IHT) and Laboratory for Emerging Nanometrology (LENA), Technische Universität Braunschweig, 38106 Braunschweig, Germany
Hutomo Suryo Wasisto
Institute of Semiconductor Technology (IHT) and Laboratory for Emerging Nanometrology (LENA), Technische Universität Braunschweig, 38106 Braunschweig, Germany
In this work we studied the influence of light emitting diode (LED) generated light on living cells which were cultivated in common cell culture microtiter plates. In detail we investigated signaling side effects including apoptosis by the use of a cell permeable peroxide activatable fluorescent dye (5,6-Chloromethyl-2′,7′-dichlorodihydrofluorescein diacetate). A high level production of peroxides in UV and blue light exposed cells was measured while the light of longer wavelengths caused only minor effects on the cells.
