Effect of trichlorphon toxin along with vitamin C on the activity of brain acetylcholinesterase, serum catalase enzymes and gill and liver tissues of Carassius auratusEffect of trichlorphon toxin along with vitamin C on the activity of brain acetylcholinesterase, serum catalase enzymes and gill and liver tissues of Carassius auratus

Abstract

The aim of this study was to evaluate the effect of trichlorfon on brain acetylcholinesterase, serum catalase enzymes, and gill and liver tissues of golden fish in the presence of vitamin C. The fish (10±0.5 g) were exposed to water-soluble trichlorfon for one month in four treatments as 1 control (no poison and vitamin), 2 (0.4 mg/l trichlorfon), 3 (0.4 mg/l trichlorfon and 200 mg/kg food vitamin C) and 4 (200 mg/kg food vitamin C), with 3 replicates. The results showed that trichlorfon significantly decreased the acetylcholinesterase activity compared to the control treatment, but the administration of vitamin C with this poison significantly (P˂0.05) increased the activity of this enzyme compared to the treatment without vitamin C. Trichlorfon did not significantly change the catalase activity compared to the control (P˃0.05). Trichlorofon caused necrosis, edema, shortness, slashing, and hyperplasia of secondary lamellas in the gills tissue of fish. This poison also caused bleeding, necrosis, and swelling of hepatocytes in the liver tissue, but because of the antioxidant properties of vitamin C, the administration of this vitamin reduced the severity of this tissue damage. According to the results, vitamin C with antioxidant properties improved enzymatic conditions and reduced the severity of gill and liver tissue damage caused by trichlorfon.

Keywords

• acetylcholinesterase
• trichlorfon
• catalase
• golden fish
• vitamin c.