Cloning, Expression, and Bioinformatics Analysis of the <i>AvFD1</i> Gene in <i>Amomum villosum</i> Lour
Duo Wang,
Yating Zhu,
Shuang Li,
Hongyou Zhao,
Chongnan Wang,
Qianxia Li,
Yanfang Wang,
Chunyong Yang,
Ge Li,
Yanqian Wang,
Lixia Zhang
Affiliations
Duo Wang
Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Jinghong 666199, China
Yating Zhu
Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Jinghong 666199, China
Shuang Li
Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Jinghong 666199, China
Hongyou Zhao
Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Jinghong 666199, China
Chongnan Wang
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100193, China
Qianxia Li
Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Jinghong 666199, China
Yanfang Wang
Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Jinghong 666199, China
Chunyong Yang
Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Jinghong 666199, China
Ge Li
Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Jinghong 666199, China
Yanqian Wang
Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Jinghong 666199, China
Lixia Zhang
Yunnan Key Laboratory of Southern Medicinal Utilization, Yunnan Branch of Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences and Peking Union Medical College, Jinghong 666199, China
As a perennial medicinal plant in the Zingiber genus, Amomum villosum Lour. faces agricultural challenges due to its prolonged vegetative and reproductive growth phases, which hinder efficient pollination and delay fruiting. To address this limitation, the present study aimed to identify the FD gene involved in regulating flowering in A. villosum to provide a basis for research on the molecular mechanisms of early fruiting cultivars. Based on the differentially expressed gene AvFD1 obtained from the transcriptome database of early fruiting plants and controls, specific primers were designed for PCR to clone the full-length sequence of AvFD1. The characteristics of the cloned AvFD1 gene were analyzed using online bioinformatics software. The expression profiles of AvFD1 in various tissues and in 1- and 2-year bearing A. villosum varieties were investigated by quantitative real-time PCR. This study successfully cloned the FD1 gene sequence of A. villosum, marking the first reported characterization of this gene in the species. Tissue-specific expression analysis revealed significantly elevated AvFD1 expression levels in stolon tips and flower buds compared to tender leaves, suggesting its potential role as a positive regulator of flowering initiation. The obtained sequence establishes essential molecular data for subsequent functional validation of AvFD1 in A. villosum.
