Preparing water-soluble n-vinylpyrrolidone-2-methyl-5-vinylpyridine n-oxide copolymers and research on their immunoadjuvant activity

Abstract

In this work partially and completely N-oxidized N-vinylpyrrolidone-2-methyl-5-vinylpyridine copolymers were synthesized. The influence of рН on the reaction course was studied. For obtaining the copolymer N-oxidized by 100% it is necessary to carry out the reaction in an acetate buffer solution. If obtaining the partially N-oxidized copolymer, a citrate buffer solution should be used, which allows carrying out the reaction at a smaller speed and as a result enables to stop the reaction in the necessary range. The dependence of biological activity on the extent of N-oxidation was studied. An immunostimulating effect of N-vinylpyrrolidone-2-methyl-5-vinylpyridine N-oxide copolymers with various extent of N-oxidation was revealed in vivo with an anthrax vaccine.

Keywords

• n-vinylpirrolidone, 2-methyl-5-vinylpyridine, n-oxide, immunoadjuvant activity.