A study on urinary isolates of Candida species isolated from hospitalized patients with special reference to speciation, antifungal susceptibility, and comparison of rapid and conventional methods of speciation

Abstract

Aim: In order to determine which species of Candida were isolated from hospitalized patients' urine, which risk factors they were linked to, and which antifungal drugs were effective against them, the current investigation was carried out. Materials and Methods: One hundred inpatients were part of the study group. Urine samples were collected from each subject and spun at 3000 rpm for 10–15 minutes in individual sterile centrifuge tubes. Color, size, texture, and the presence of color diffusion into the surrounding agar were used to identify the distinct Candida species, presumably in 48 hours. Results: A total of 86.7% of the Candida spp. were found to be non-albicans. Major inaccuracies occurred because of differences between methodologies; thus, MBD is the best way to determine antifungal susceptibility to itraconazole and amphotericin B. Conclusion: To help clinicians decide on an empirical treatment in an emergency, accurate identification of Candida down to the species level is crucial.

Keywords

• antifungal susceptibility
• candida species
• urine