Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

Demetra S.M. Chatzileontiadou; Christopher Szeto; Dhilshan Jayasinghe; Stephanie Gras

STAR Protocols (Sep 2021)

Protein purification and crystallization of HLA-A∗02:01 in complex with SARS-CoV-2 peptides

Demetra S.M. Chatzileontiadou,
Christopher Szeto,
Dhilshan Jayasinghe,
Stephanie Gras

Affiliations

Demetra S.M. Chatzileontiadou: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC 3083, Australia; Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia; Corresponding author
Christopher Szeto: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC 3083, Australia; Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia
Dhilshan Jayasinghe: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC 3083, Australia; Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia
Stephanie Gras: Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC 3083, Australia; Department of Biochemistry and Molecular Biology, Monash University, Clayton, VIC 3800, Australia; Corresponding author

Journal volume & issue: Vol. 2, no. 3
p. 100635

Abstract

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Summary: Understanding T-cell responses requires identifying viral peptides presented by human leukocyte antigens (HLAs). X-ray crystallography can be used to visualize their presentation. This protocol describes the expression, purification, and crystallization of HLA-A∗02:01, one of the most frequent HLA in the global population in complex with peptides derived from the SARS-CoV-2 nucleocapsid protein. This protocol can be applied to different HLA class I molecules bound to other peptides.For complete details on the use and execution of this protocol, please refer to Szeto et al. (2021).

Published in STAR Protocols

ISSN: 2666-1667 (Online)
Publisher: Elsevier
Country of publisher: United States
LCC subjects: Science: Science (General)
Website: https://star-protocols.cell.com/

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