Plackett-Burman design in robustness testing of liquid chromatographic method for determination of sodium-valproat

Abstract

In this paper, application of Plackett-Burman design in robustness testing of liquid chromatographic method for determination of sodium valproate. As factors which could have influence on method's robustness content of acetonitrile, concentration of sodium dihydrogenphosphate, pH of the mobile phase, column's temperature, flow rate and wavelenght were investigated. In aim to create plan of experiments for Plackett-Burman design on six real factors, 5 dummies were added. In total, 11 factors were investigated throught 12 experiments. As qualitative responses retention times, number of theoretical plates and peak symmetry were followed while as quantitative response peak area was followed. On the basis of the obtained results with statistical and graphical methods, influence of investigated factors on system responses were defined. Further, for significant factors non-significant interval was defined e. g. range in which factor could be change without influence on response. Finally, results were used for determination of parameters for system suitability tests.

Keywords

• plackett-burman design
• robustness
• sodium valproate
• liquid chromatography