A Novel Immunoassay for Malondialdehyde-Conjugated Low-Density Lipoprotein Measures Dynamic Changes in the Blood of Patients Undergoing Coronary Artery Bypass Graft Surgery
Samata S. Pandey,
Adam Hartley,
Mikhail Caga-Anan,
Tareq Ammari,
Ameer Hamid Ahmed Khan,
Bao Anh Vu Nguyen,
Chiari Kojima,
Jon Anderson,
Steven Lynham,
Michael Johns,
Dorian O. Haskard,
Ramzi Y. Khamis
Affiliations
Samata S. Pandey
Vascular Sciences Section, National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College London, London W12 0NN, UK
Adam Hartley
Vascular Sciences Section, National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College London, London W12 0NN, UK
Mikhail Caga-Anan
Vascular Sciences Section, National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College London, London W12 0NN, UK
Tareq Ammari
Vascular Sciences Section, National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College London, London W12 0NN, UK
Ameer Hamid Ahmed Khan
Vascular Sciences Section, National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College London, London W12 0NN, UK
Bao Anh Vu Nguyen
Vascular Sciences Section, National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College London, London W12 0NN, UK
Chiari Kojima
Vascular Sciences Section, National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College London, London W12 0NN, UK
Jon Anderson
Department of Cardiothoracic Surgery, Hammersmith Hospital, Imperial College Healthcare NHS Trust, London W12 0HS, UK
Steven Lynham
Centre of Excellence for Mass Spectrometry, Proteomics Facility, Denmark Hill Campus, Kings College London, London SE5 9NU, UK
Michael Johns
Vascular Sciences Section, National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College London, London W12 0NN, UK
Dorian O. Haskard
Vascular Sciences Section, National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College London, London W12 0NN, UK
Ramzi Y. Khamis
Vascular Sciences Section, National Heart and Lung Institute, Imperial Centre for Translational and Experimental Medicine, Imperial College London, London W12 0NN, UK
Oxidized low-density lipoproteins play an important role in tissue pathology. In this study, we report a sensitive novel enzyme-linked immunosorbent assay for the detection of malondialdehyde-modified low-density lipoprotein (MDA-LDL), a key component of oxidized LDL. The assay is capable of measuring a variable presence of MDA-LDL within human plasma and serum. We demonstrate the robust nature of the assay on samples stored for over 20 months, as well as high inter-operator reproducibility (r = 0.74, p < 0.0001). The assay was capable of detecting dynamic changes in patient blood samples after coronary artery bypass graft surgery, indicating synthesis or release of MDA-LDL with the oxidative stress of surgery, followed by homeostatic clearance. This robust, sensitive and specific assay for circulating MDA-LDL will serve as a valuable translational tool for the improved detection of oxidative forms of LDL in response to a range of physiological or pathological stimuli, with potential clinical applicability.